使用共聚焦显微镜对正常雌性实验比格犬的角膜进行体内眼部成像。
In vivo ocular imaging of the cornea of the normal female laboratory beagle using confocal microscopy.
作者信息
Strom Ann R, Cortés Dennis E, Thomasy Sara M, Kass Philip H, Mannis Mark J, Murphy Christopher J
机构信息
Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Department of Ophthalmology & Vision Science, UC Davis Eye Center, School of Medicine, University of California, Davis, CA, 95616, USA.
出版信息
Vet Ophthalmol. 2016 Jan;19(1):63-7. doi: 10.1111/vop.12264. Epub 2015 Mar 6.
OBJECTIVE
To obtain normative data for the normal laboratory beagle cornea using high-resolution in vivo confocal microscopy (IVCM).
ANIMALS STUDIED
Sixteen eyes of eight healthy young female intact beagles.
PROCEDURES
The central cornea was imaged using IVCM. Mixed effects linear regression was used for statistical analysis.
RESULTS
in vivo confocal microscopy allowed detailed visualization and quantification of epithelial cells (superficial epithelial cell diameter: 43.25 ± 6.64 μm, basal cell diameter: 4.43 ± 0.67 μm), and nerve fibers (subepithelial nerve fiber diameter: 2.38 ± 0.69 μm, anterior stromal nerve fiber diameter: 16.93 ± 4.55 μm). Keratocyte density (anterior stroma 993.38 ± 134.24 cells/mm(2) , posterior stroma 789.38 ± 87.13 cells/mm(2) ) and endothelial cell density (2815.18 ± 212.59 cells/mm(2) ) were also measured.
CONCLUSION
High-resolution IVCM provides detailed noninvasive evaluation of the cornea in the normal laboratory beagle.
目的
使用高分辨率活体共聚焦显微镜（IVCM）获取正常实验用比格犬角膜的标准数据。
研究动物
8只健康年轻未绝育雌性比格犬的16只眼睛。
方法
使用IVCM对中央角膜进行成像。采用混合效应线性回归进行统计分析。
结果
活体共聚焦显微镜能够详细观察和量化上皮细胞（表层上皮细胞直径：43.25±6.64μm，基底细胞直径：4.43±0.67μm）和神经纤维（上皮下神经纤维直径：2.38±0.69μm，前基质神经纤维直径：16.93±4.55μm）。还测量了角膜细胞密度（前基质993.38±134.24个细胞/mm²，后基质789.38±87.13个细胞/mm²）和内皮细胞密度（2815.18±212.59个细胞/mm²）。
结论
高分辨率IVCM可对正常实验用比格犬的角膜进行详细的无创评估。
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