Lieth E, Towle A C, Lauder J M
Dept. of Cell Biology and Anatomy, School of Medicine, University of North Carolina, Chapel Hill 27599-7090.
Neurochem Res. 1989 Oct;14(10):979-85. doi: 10.1007/BF00965932.
Effects of astroglia on the morphological and biochemical differentiation of catecholamine neurons from embryonic rat mesencephalon were studied in vitro, and compared to results obtained with fibroblasts. Neurite outgrowth and complexity were measured using computer-assisted morphometry on tyrosine hydroxylase immunoreactive neurons growing on preformed monolayers of astrocytes or fibroblasts. The morphological differentiation of these neurons was stimulated by the presence of astrocytes, and this effect was evident in various cellular compartments, including the size of the cell soma, length of neurites and neuritic segments, and the numbers of these segments. Tyrosine hydroxylase activity was measured biochemically in these cultures and was also found to be stimulated by the presence of astroglial monolayers. The implication of these results for the understanding of specific neuron-glial interactions during embryonic brain development is discussed.
研究了星形胶质细胞对体外培养的胚胎大鼠中脑儿茶酚胺能神经元形态和生化分化的影响，并与成纤维细胞的实验结果进行了比较。使用计算机辅助形态测量法，对生长在星形胶质细胞或成纤维细胞预形成单层上的酪氨酸羟化酶免疫反应性神经元的神经突生长和复杂性进行了测量。这些神经元的形态分化受到星形胶质细胞的刺激，这种作用在各个细胞区室中都很明显，包括细胞体大小、神经突和神经突节段的长度以及这些节段的数量。通过生化方法测量了这些培养物中的酪氨酸羟化酶活性，发现其也受到星形胶质细胞单层的刺激。讨论了这些结果对理解胚胎脑发育过程中特定神经元-胶质细胞相互作用的意义。
