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杂色曲霉在模拟废水和半合成培养基中对农药三氯生的生物降解作用

Biodegradation of pesticide triclosan by A. versicolor in simulated wastewater and semi-synthetic media.

作者信息

Ertit Taştan Burcu, Dönmez Gönül

机构信息

Department of Biology, Faculty of Science, Ankara University, 06100, Beşevler, Ankara, Turkey; Life Sciences Application and Research Center, Gazi University, 06830, Gölbaşı, Ankara, Turkey; Health Services Vocational School, Gazi University, 06830, Gölbaşı, Ankara, Turkey.

Department of Biology, Faculty of Science, Ankara University, 06100, Beşevler, Ankara, Turkey.

出版信息

Pestic Biochem Physiol. 2015 Feb;118:33-7. doi: 10.1016/j.pestbp.2014.11.002. Epub 2014 Nov 11.

DOI:10.1016/j.pestbp.2014.11.002
PMID:25752427
Abstract

Triclosan is known as an antimicrobial agent, a powerful bacteriostat and an important pesticide. In this paper biodegradation of triclosan by Aspergillus versicolor was investigated. Effects of simulated wastewater and semi-synthetic media on fungal triclosan degradation process were detected. HPLC analysis showed that fungal triclosan biodegradation yield was 71.91% at about 7.5 mg/L concentration in semi-synthetic medium and was 37.47% in simulated wastewater. Fungus could be able to tolerate the highest triclosan concentration (15.69 mg/L). The biodegradation yield was 29.81% and qm was 2.22 mg/g at this concentration. Some of the parameters, such as pH, culture media, increasing triclosan and biomass concentrations were optimized in order to achieve the effective triclosan biodegradation process. The highest triclosan biodegradation yields of all microorganisms were achieved by A. versicolor.

摘要

三氯生是一种抗菌剂、强效抑菌剂和重要的杀虫剂。本文研究了杂色曲霉对三氯生的生物降解作用。检测了模拟废水和半合成培养基对真菌三氯生降解过程的影响。高效液相色谱分析表明,在半合成培养基中,三氯生浓度约为7.5mg/L时,真菌对三氯生的生物降解率为71.91%,在模拟废水中为37.47%。真菌能够耐受的最高三氯生浓度为15.69mg/L。在此浓度下,生物降解率为29.81%,qm为2.22mg/g。为了实现有效的三氯生生物降解过程,对一些参数进行了优化,如pH值、培养基、增加三氯生和生物量浓度等。杂色曲霉实现了所有微生物中最高的三氯生生物降解率。

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