Eisses K T
Biochemical Institute, University of Oslo, Norway.
Teratog Carcinog Mutagen. 1989;9(5):315-25. doi: 10.1002/tcm.1770090506.
Ethylene glycol monomethyl ether (EGME) or 2-methoxyethanol (2-ME) is a toxic compound that has teratogenic effects in developing organisms similar to its oxidation product methoxyacetic acid (MAA). Alcohol dehydrogenase (ADH) is supposed to be involved with the oxidation of EGME into MAA. It has been shown here that Drosophila ADH dehydrogenates EGME in an in vitro test system. Three strains of Drosophila melanogaster, genetically different from each other with regard to ADH activities (qualitatively and quantitatively), were used to test the in vivo involvement of ADH with the oxidation of EGME into MAA. The toxicity of EGME in egg-to-adult and third instar larval-to-adult development is concentration and strain dependent. Higher detoxification occurs in the strain with increased ADH activity. EGME is much more toxic than its oxidation product MAA at the level of adult eclosion. Teratogenic effects were observed in the ADH-negative strain in spite of lacking ADH activity. EGME is apparently a teratogenic compound by itself.
乙二醇单甲醚（EGME）或2-甲氧基乙醇（2-ME）是一种有毒化合物，在发育中的生物体中具有致畸作用，类似于其氧化产物甲氧基乙酸（MAA）。乙醇脱氢酶（ADH）被认为参与了EGME氧化为MAA的过程。本文已表明，果蝇ADH在体外测试系统中可使EGME脱氢。使用了三株黑腹果蝇，它们在ADH活性（定性和定量）方面彼此基因不同，以测试ADH在体内是否参与EGME氧化为MAA的过程。EGME在卵到成虫以及三龄幼虫到成虫发育过程中的毒性取决于浓度和品系。ADH活性增加的品系中解毒作用更强。在成虫羽化水平上，EGME比其氧化产物MAA毒性大得多。尽管缺乏ADH活性，但在ADH阴性品系中仍观察到致畸作用。显然，EGME本身就是一种致畸化合物。
