Lauharanta J, Salonen E M, Vaheri A
Department of Dermatology, University Central Hospital, Helsinki, Finland.
Acta Derm Venereol. 1989;69(6):527-9.
Blister fluid from tense bullae of 10 patients with bullous pemphigoid was investigated using a radial caseinolysis assay and zymography. Proteolytic activity, varying from 4.7 to 10 micrograms/ml, was found in 3 out of 10 patients, by using the caseinolysis assay. Zymography revealed that a major part of this caseinolytic activity co-migrated with plasmin standard. In addition, in the zymography, proteolytically active high molecular weight complexes were seen. This characteristic pattern was seen in the zymography of both positive and negative samples in the caseinolysis assay. These high molecular weight complexes were not seen in the zymography of the blister fluid of 2 patients with epidermolysis bullosa or in the suction blister fluid of 3 healthy control patients. These findings suggest that plasmin is generated at some phase of the blister formation, being possibly involved in the pathomechanism of bullous pemphigoid.
使用放射状酪蛋白溶解试验和酶谱分析法对10例大疱性类天疱疮患者紧张性大疱的疱液进行了研究。通过酪蛋白溶解试验，在10例患者中有3例发现蛋白水解活性，范围为4.7至10微克/毫升。酶谱分析显示，这种酪蛋白溶解活性的主要部分与纤溶酶标准品共迁移。此外，在酶谱分析中，可见蛋白水解活性的高分子量复合物。在酪蛋白溶解试验的阳性和阴性样本的酶谱分析中均可见到这种特征性模式。在2例大疱性表皮松解症患者的疱液或3例健康对照患者的抽吸疱液的酶谱分析中未见到这些高分子量复合物。这些发现表明，纤溶酶在水疱形成的某个阶段产生，可能参与大疱性类天疱疮的发病机制。
