Guo Qian-Qian, Ming Hong, Meng Xiao-Ling, Duan Yan-Yan, Gao Rui, Zhang Jian-Xin, Huang Jian-Rong, Li Wen-Jun, Nie Guo-Xing
College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 May;107(5):1271-80. doi: 10.1007/s10482-015-0421-7. Epub 2015 Mar 10.
A Gram-positive, aerobic, non-motile and non-spore forming strain, designated CFH S0262(T), was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. A polyphasic approach was used to study the taxonomic position of this new isolate. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain CFH S0262(T) belongs to the genus Rhodococcus and clustered with Rhodococcus soli DSD51W(T), Rhodococcus hoagii NBRC 103062(T), Rhodococcus defluvii Call(T) and Rhodococcus kunmingensis YIM 45607(T) (98.7, 98.5, 97.9 and 97.6% similarities, respectively). Strain CFH S0262(T) could grow in the presence of NaCl (0-4%, optimum 0-3%), at pH 6.0-8.0 (optimum, pH 7.0) and at 10-40 °C (optimum, 28 °C). The predominant menaquinones of strain CFH S0262(T) were identified as MK-8 (H2) and MK-8 (H4). The major fatty acids (≥10%) were found to be C(16:0) and C(18:1)ω9c. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, a glycolipid and two unidentified phospholipids. The DNA G+C content was determined to be 71.4 mol%. Based on a comparative analysis of phenotypic and genotypic characteristics, in combination with low values of DNA-DNA hybridization between strain CFH S0262(T) and its closest neighbours, it is proposed that strain CFH S0262(T) represents a novel species of the genus Rhodococcus, for which the name Rhodococcus agglutinans sp. nov., is proposed, with the type strain CFH S0262(T) (=CCTCC AB2014297(T)=KCTC 39118(T)).
从越南下龙湾吉婆岛采集的土壤样本中分离出一株革兰氏阳性、需氧、不运动且不产芽孢的菌株，命名为CFH S0262(T)。采用多相分类法研究这一新分离株的分类地位。基于16S rRNA基因序列的系统发育分析表明，菌株CFH S0262(T)属于红球菌属，与土壤红球菌DSD51W(T)、霍氏红球菌NBRC 103062(T)、污水红球菌Call(T)和昆明红球菌YIM 45607(T)聚类在一起（相似性分别为98.7%、98.5%、97.9%和97.6%）。菌株CFH S0262(T)在NaCl存在的情况下（0 - 4%，最适0 - 3%）、pH 6.0 - 8.0（最适pH 7.0）以及10 - 40 °C（最适28 °C）条件下能够生长。菌株CFH S0262(T)的主要甲基萘醌被鉴定为MK - 8(H2)和MK - 8(H4)。发现主要脂肪酸（≥10%）为C(16:0)和C(18:1)ω9c。检测到的极性脂质有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种糖脂和两种未鉴定的磷脂。DNA G + C含量测定为71.4 mol%。基于表型和基因型特征的比较分析，结合菌株CFH S0262(T)与其最接近亲缘种之间较低的DNA - DNA杂交值，建议菌株CFH S0262(T)代表红球菌属的一个新物种，命名为凝集红球菌（Rhodococcus agglutinans）新种，模式菌株为CFH S0262(T)（=CCTCC AB2014297(T)=KCTC 39118(T)）。
