Department of Science Education, Jeju National University, Jeju 63243, Republic of Korea.
Present address: R&D Center, BioPS Co., Ltd., Daedeuk Valley Campus, Hannam University, Daejon 34054, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3128-3134. doi: 10.1099/ijsem.0.003601.
A novel Gram-stain-positive actinobacterial strain, designated C9-28, was isolated from soil sampled in a natural cave on Jeju Island, Republic of Korea. Strain C9-28 morphologically exhibited a rod-coccus life cycle and grew at 10-37 °C (optimum, 30 °C), pH 6-9 (optimum, pH 7) and 0-3 % (optimum, absence of NaCl). In the maximum-likelihood tree based on 16S rRNA gene sequences, strain C9-28 formed a sublineage between a clade and the type strain of . The closest relatives of strain C9-28 were the type strains of (98.88 % 16S rRNA gene sequence similarity), (98.88 %) and (98.60 %). The phylogenomic tree based on whole genome sequences supported the distinct position of the novel strain within the genus . The following chemotaxonomic characteristics also supported the assignment to the genus: -diaminopimelic acid; arabinose and galactose in whole-cell hydrolysates; the predominant menaquinone of MK-8(H); and polar lipids including diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, three unidentified glycolipids and two unidentified lipids. The predominant cellular fatty acids were C, summed feature 3 (Cω7 and/or Cω6), Cω9 and C. Based on the values of average nucleotide identity and digital DNA-DNA hybridization from whole genome sequences, and DNA-DNA hybridization between the isolate and the closest relatives, strain C9-28 (=KACC 19823=DSM 107559) represents a novel species of the genus , for which the name sp. nov. is proposed.
一株新型革兰氏阳性放线菌菌株，命名为 C9-28，从韩国济州岛天然洞穴土壤中分离得到。菌株 C9-28 在形态上表现出杆-球菌的生命周期，生长温度为 10-37°C（最佳温度为 30°C），pH 值为 6-9（最佳 pH 值为 7），0-3%（最佳为无 NaCl）。在基于 16S rRNA 基因序列的最大似然树中，菌株 C9-28 形成了一个分支和 模式菌株之间的亚分支。菌株 C9-28 的最接近的亲缘关系是菌株 （98.88%的 16S rRNA 基因序列相似性）、 （98.88%）和 （98.60%）。基于全基因组序列的系统发育树支持该新型菌株在属内的独特位置。以下化学分类特征也支持其在属内的归属：-二氨基庚二酸；全细胞水解物中的阿拉伯糖和半乳糖；主要的menaquinone 为 MK-8(H)；极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、三种未鉴定的糖脂和两种未鉴定的脂类。主要的细胞脂肪酸为 C 、特征 3 总和（Cω7 和/或 Cω6）、Cω9 和 C 。基于全基因组序列的平均核苷酸同一性和数字 DNA-DNA 杂交值，以及菌株与最接近亲缘体之间的 DNA-DNA 杂交值，菌株 C9-28（=KACC 19823=DSM 107559）代表了属的一个新型种，建议命名为 sp. nov.。
