Damerell David, Ceroni Alessio, Maass Kai, Ranzinger René, Dell Anne, Haslam Stuart M
Department of Life Sciences, Faculty of Natural Sciences, Sir Ernst Chain Building, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.
Methods Mol Biol. 2015;1273:3-15. doi: 10.1007/978-1-4939-2343-4_1.
The GlycoWorkbench software tool allows users to semiautomatically annotate glycomics MS and MS/MS spectra and MS glycoproteomics spectra. The GlycanBuilder software tool is embedded within GlycoWorkbench allowing users to draw glycan structures and export images of the drawn structures. This chapter demonstrates to users how to draw glycan structures within GlycoWorkbench using the GlycanBuilder software tool. This chapter also demonstrates how to use GlycoWorkbench to import MS and MS/MS glycomics spectra and use the cascading annotation feature to annotate both the MS and MS/MS spectra with a single command.
GlycoWorkbench软件工具允许用户对糖组学质谱和串联质谱光谱以及质谱糖蛋白质组学光谱进行半自动注释。GlycanBuilder软件工具嵌入在GlycoWorkbench中，允许用户绘制聚糖结构并导出所绘制结构的图像。本章向用户演示如何使用GlycanBuilder软件工具在GlycoWorkbench中绘制聚糖结构。本章还演示了如何使用GlycoWorkbench导入质谱和串联质谱糖组学光谱，并使用级联注释功能通过单个命令对质谱和串联质谱光谱进行注释。
