Li Xiaoxu, Zhang Xiaohua, Yao Rujiao, He Yang, Zhu Yongyong, Qian Jie
School of Mechanical and Electrical Engineering, Soochow University, Suzhou, 215021, China,
J Am Soc Mass Spectrom. 2015 May;26(5):734-40. doi: 10.1007/s13361-015-1085-2. Epub 2015 Mar 10.
A novel linear ion trap mass analyzer featuring half round rod electrodes (HreLIT) has been built. It is mainly composed of two pairs of stainless steel electrodes which have a cross-section of half round rod and a pair of end electrodes. The HreLIT has a simple structure and so it could be assembled by hand with relatively high mechanical accuracy. The external dimension of HreLIT is 50 mm × 29.5 mm × 28 mm (length × width × height) and its internal volume is about 3.8 cm(3). A home-made HreLIT mass spectrometer with three-stage vacuum system was built and the performance of HreLIT was characterized using reserpine solution and PPG standard solution. When the scan rate was 254 u/s, mass peak with FWHM of 0.14 u was achieved for ions with m/z 609, which corresponds to a mass resolution of 4350. The HreLIT was also operated at a low q value of 0.28 to extend its mass range. The experiment result showed a mass range of over 2800 u and the amplitude of radio frequency (rf) signal was only 1560 V (0-p). Three-stage tandem mass spectrometry was successfully performed in the HreLIT, and the collision-induced dissociation (CID) efficiencies of MS(2) (CID of ions with m/z 609) and MS(3) (CID of ions with m/z 448) were 78% and 59%, respectively.
一种具有半圆形杆电极的新型线性离子阱质量分析仪（HreLIT）已被制造出来。它主要由两对横截面为半圆形杆的不锈钢电极和一对端电极组成。HreLIT结构简单，因此可以手工组装，且机械精度相对较高。HreLIT的外部尺寸为50毫米×29.5毫米×28毫米（长×宽×高），其内部体积约为3.8立方厘米。构建了一台带有三级真空系统的自制HreLIT质谱仪，并使用利血平溶液和PPG标准溶液对HreLIT的性能进行了表征。当扫描速率为254 u/s时，对于质荷比为609的离子，获得了半高宽为0.14 u的质量峰，这对应于4350的质量分辨率。HreLIT还在0.28的低q值下运行以扩展其质量范围。实验结果表明质量范围超过2800 u，射频（rf）信号的幅度仅为1560 V（0至峰值）。在HreLIT中成功进行了三级串联质谱分析，MS（2）（质荷比为609的离子的碰撞诱导解离（CID））和MS（3）（质荷比为448的离子的CID）的碰撞诱导解离效率分别为78%和59%。
