Huang Tao, Chen Xiaoying, Hong Qingxiao, Deng Zaichun, Ma Hongying, Xin Yanfei, Fang Yong, Ye Huadan, Wang Rujie, Zhang Cheng, Ye Meng, Duan Shiwei
1] Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China [2] The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315020, China.
Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
Sci Rep. 2015 Mar 10;5:8897. doi: 10.1038/srep08897.
Aberrant DNA methylation can be a potential genetic mechanism in non-small cell lung cancer (NSCLC). However, inconsistent findings existed among the recent association studies between cigarette smoking and gene methylation in lung cancer. The purpose of our meta-analysis was to evaluate the role of gene methylation in the smoking behavior of NSCLC patients. A total of 116 genes were obtained from 97 eligible publications in the current meta-analyses. Our results showed that 7 hypermethylated genes (including CDKN2A, RASSF1, MGMT, RARB, DAPK, WIF1 and FHIT) were significantly associated with the smoking behavior in NSCLC patients. The further population-based subgroup meta-analyses showed that the CDKN2A hypermethylation was significantly associated with cigarette smoking in Japanese, Chinese and Americans. In contrast, a significant association of RARB hypermethylation and smoking behavior was only detected in Chinese but not in Japanese. The genes with altered DNA methylation were likely to be potentially useful biomarkers in the early diagnosis of NSCLC.
异常的DNA甲基化可能是非小细胞肺癌（NSCLC）的一种潜在遗传机制。然而，近期关于吸烟与肺癌基因甲基化的关联研究结果并不一致。我们进行荟萃分析的目的是评估基因甲基化在NSCLC患者吸烟行为中的作用。在当前的荟萃分析中，从97篇符合条件的出版物中获取了总共116个基因。我们的结果表明，7个高甲基化基因（包括CDKN2A、RASSF1、MGMT、RARB、DAPK、WIF1和FHIT）与NSCLC患者的吸烟行为显著相关。进一步基于人群的亚组荟萃分析表明，CDKN2A高甲基化在日本人、中国人和美国人中与吸烟显著相关。相比之下，仅在中国人群中检测到RARB高甲基化与吸烟行为存在显著关联，而在日本人群中未检测到。DNA甲基化改变的基因可能是NSCLC早期诊断中潜在有用的生物标志物。
