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采用 ELISA 试剂盒对中国市场农产品中脱氧雪腐镰刀菌烯醇污染的调查。

Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit.

机构信息

Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.

Institute of Plant Protection and Agro-Product Safety, Anhui Academy of Agricultural Sciences, Key Laboratory of Agro-Product Safety Risk Evaluation (Hefei), Ministry of Agriculture, South Road of agricultural science 40, Hefei 230031, China.

出版信息

Toxins (Basel). 2018 Dec 24;11(1):6. doi: 10.3390/toxins11010006.

DOI:10.3390/toxins11010006
PMID:30586927
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6357168/
Abstract

A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL limit of detection (LOD) in working buffer and a 200 ng g LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g, and the highest DON contamination was found in distillers' dried grains with solubles with an average of 3204.5 ng g. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health.

摘要

本研究采用自行研制的酶联免疫吸附测定法（ELISA）试剂盒，对来自中国各地面粉厂、饲料厂和养殖场的 328 份高度疑似受污染的农产品样本进行脱氧雪腐镰刀菌烯醇（DON）污染检测。该试剂盒在工作缓冲液中的检测限（LOD）为 4.9ng mL，在真实样品中的 LOD 为 200ng g。DON 污染检出率为 88.7%，浓度范围为 200.9-6480.6ng g，最高污染水平出现在酒糟中，平均含量为 3204.5ng g。小麦麸皮和小麦是最常见的受污染样品，玉米粉样品的 DON 平均水平最低。该 ELISA 试剂盒是一种快速、灵敏、特异、准确、高通量测定 DON 的替代方法，可以满足最大限量要求。本研究表明，DON 在中国市场的污染较为严重，污染风险值得关注。应采取必要的预防措施，确保食品安全和人类健康。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/f5a1d0b161ce/toxins-11-00006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/a41f775caab7/toxins-11-00006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/6d269693f98e/toxins-11-00006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/c37cc5ceafc7/toxins-11-00006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/f5a1d0b161ce/toxins-11-00006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/a41f775caab7/toxins-11-00006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/6d269693f98e/toxins-11-00006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/c37cc5ceafc7/toxins-11-00006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2345/6357168/f5a1d0b161ce/toxins-11-00006-g004.jpg

相似文献

[1]
Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit.

Toxins (Basel). 2018-12-24

[2]
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[3]
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[4]
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[5]
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[6]
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[7]
[Pollution investigation of deoxynivalenol in wheat flour of China in 2013].

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[8]
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[10]
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引用本文的文献

[1]
mycotoxins: The major food contaminants.

mLife. 2024-5-13

[2]
Ameliorative Effects of Luteolin and Activated Charcoal on Growth Performance, Immunity Function, and Antioxidant Capacity in Broiler Chickens Exposed to Deoxynivalenol.

Toxins (Basel). 2023-7-26

[3]
Comparison of Synthetic Methods and Identification of Several Artificial Antigens of Deoxynivalenol.

Molecules. 2023-3-20

[4]
Providing Biological Plausibility for Exposure-Health Relationships for the Mycotoxins Deoxynivalenol (DON) and Fumonisin B1 (FB1) in Humans Using the AOP Framework.

Toxins (Basel). 2022-4-13

[5]
Nontoxic-dose deoxynivalenol aggravates lipopolysaccharides-induced inflammation and tight junction disorder in IPEC-J2 cells through activation of NF-κB and LC3B.

Food Chem Toxicol. 2020-11

[6]
Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.

Molecules. 2019-12-22

[7]
Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016-2018 as Assessed by Magnetic Bead Immunoassay.

Toxins (Basel). 2019-8-1

本文引用的文献

[1]
Simultaneous determination of aflatoxin B, fumonisin B and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor.

J Hazard Mater. 2018-7-23

[2]
Deoxynivalenol, gut microbiota and immunotoxicity: A potential approach?

Food Chem Toxicol. 2018-1-10

[3]
Highly efficient detection of salbutamol in environmental water samples by an enzyme immunoassay.

Sci Total Environ. 2017-9-21

[4]
Sensitive immunoassay for simultaneous determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether: An effective and reliable strategy to estimate the typical tetrabromobisphenol A derivative and byproduct in aquatic environments.

Environ Pollut. 2017-10

[5]
Time-resolved immunoassay based on magnetic particles for the detection of diethyl phthalate in environmental water samples.

Sci Total Environ. 2017-6-1

[6]
The Antagonistic Effect of Mycotoxins Deoxynivalenol and Zearalenone on Metabolic Profiling in Serum and Liver of Mice.

Toxins (Basel). 2017-1-10

[7]
Group detection of DON and its modified forms by an ELISA kit.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017-2

[8]
Highly efficient detection of paclobutrazol in environmental water and soil samples by time-resolved fluoroimmunoassay.

Sci Total Environ. 2016-6-16

[9]
A universal multi-wavelength fluorescence polarization immunoassay for multiplexed detection of mycotoxins in maize.

Biosens Bioelectron. 2015-12-17

[10]
Enzyme-linked immunosorbent assay for triclocarban in aquatic environments.

Water Sci Technol. 2015

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