Wabaidur Saikh mohammad, Alam Seikh Mafiz, Alothman Zeid A, Mohsin Kazi
Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Chemistry, Aliah University, West Bengal, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jun 5;144:170-5. doi: 10.1016/j.saa.2015.02.051. Epub 2015 Feb 21.
Silver nanoparticles have been utilized for the enhanced chemiluminogenic estimation of fluoroquinolone antibiotic gatifloxacin. It has been found that the weak chemiluminescence intensity produced from the reaction between calcein and KMnO4 can further be strengthened by the addition of silver nanoparticles in the presence of gatifloxacin. This phenomenon has been exploited to the quantitative determination of gatifloxacin. Under the optimum experimental conditions, the calibration curves are linear over the range of 8.9×10(-9)-4.0×10(-6) M, while the limits of detections were found to be 2.6×10(-9) M with correlation coefficient value (r(2)) 0.9999. The relative standard deviation calculated from six replicate measurements (1.0×10(-4) M gatifloxacin) was 1.70%. The method was applied to pharmaceutical preparations and the results obtained were in reasonable agreement with the amount labeled on the formulations. The proposed method was also used for the determination of gatifloxacin in spiked urine samples with satisfactory results. No interference effects from some common excipients used in pharmaceutical preparations have been found.
银纳米颗粒已被用于增强氟喹诺酮类抗生素加替沙星的化学发光测定。研究发现，在加替沙星存在的情况下，加入银纳米颗粒可进一步增强钙黄绿素与高锰酸钾反应产生的微弱化学发光强度。这一现象已被用于加替沙星的定量测定。在最佳实验条件下，校准曲线在8.9×10(-9)-4.0×10(-6) M范围内呈线性，检测限为2.6×10(-9) M，相关系数值（r(2)）为0.9999。由六次重复测量（1.0×10(-4) M加替沙星）计算得到的相对标准偏差为1.70%。该方法应用于药物制剂，所得结果与制剂上标注的含量合理一致。所提出的方法还用于加标尿样中加替沙星的测定，结果令人满意。未发现药物制剂中一些常见辅料的干扰效应。
