Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Chemistry, Aliah University, Kolkata, West Bengal, India.
IET Nanobiotechnol. 2018 Sep;12(6):773-780. doi: 10.1049/iet-nbt.2018.0018.
The flow injection combined with tris-(bipyridyl) ruthenium (II)-cerium (IV) chemiluminescence (CL) method based on the sensitisation of silver nanoparticles (AgNPs) has been established for the quantitative analysis of gatifloxacin (GFX). AgNPs were synthesised using the reaction between ammonia gas and silver nitrate solution and was used to increase the CL intensity of the proposed system. The enhancement of CL intensity was linear with the concentration of GFX added. Effects of different experimental parameters were studied. Under the optimal conditions, the linear relationship was established between the concentration range of 1.4 × 10-4.5 × 10 M, with the correlation coefficient (r) 02E9999. The limit of detection was found to be 4.6 × 10 M. The relative standard deviation obtained was 3.2% for six replicate measurements of GFX (1.2 × 10 M). The proposed CL method was applied to the commercial drug and the result was in agreement with the labelled amount. The technique was further adopted to the analysis of GFX in spiked urine samples. Negligible interference was found (variation in CL intensity <5%) from a few common foreign excipients applied in preparation of pharmaceutical drug. The comparative results with a few reported methods indicates that the proposed method is more sensitive than other methods..
基于银纳米粒子 (AgNPs) 敏化的三（联吡啶）钌 (II)-铈 (IV) 化学发光 (CL) 与流动注射联用，建立了用于定量分析加替沙星 (GFX) 的方法。AgNPs 是通过氨气体与硝酸银溶液之间的反应合成的，并用于增强所提出体系的 CL 强度。CL 强度的增强与添加的 GFX 浓度呈线性关系。研究了不同实验参数的影响。在最佳条件下，建立了浓度范围为 1.4×10-4.5×10 M 的线性关系，相关系数 (r) 为 0.9999。检测限为 4.6×10 M。对于 GFX（1.2×10 M）的六个重复测量，获得的相对标准偏差为 3.2%。该建议的 CL 方法被应用于商业药物，结果与标签数量一致。该技术进一步用于加替沙星在掺杂尿样中的分析。从用于制备药物的几种常见的外来赋形剂中，发现 CL 强度的变化可忽略不计（<5%）。与一些已报道的方法的比较结果表明，该方法比其他方法更灵敏。
