Park Sooyeon, Park Ji-Min, Kang Chul-Hyung, Kim Song-Gun, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1819-1824. doi: 10.1099/ijs.0.000182. Epub 2015 Mar 9.
A Gram-stain-negative, non-motile, aerobic and pleomorphic bacterium, designated BS-W13T, was isolated from a tidal flat on the South Sea, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BS-W13T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain BS-W13T clustered with the type strain of Seohaeicola saemankumensis, showing the highest sequence similarity (95.96 %) to this strain. Strain BS-W13T exhibited 16S rRNA gene sequence similarity values of 95.95, 95.91, 95.72 and 95.68 % to the type strains of Sulfitobacter donghicola, Sulfitobacter porphyrae, Sulfitobacter mediterraneus and Roseobacter litoralis, respectively. Strain BS-W13T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The polar lipid profile of strain BS-W13T, containing phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid as major components, was distinguishable from those of some phylogenetically related taxa. The DNA G+C content of strain BS-W13T was 58.1 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain BS-W13T constitutes a novel genus and species within family Rhodobacteraceae of the class Alphaproteobacteria, for which the name Pseudoseohaeicola caenipelagi gen. nov., sp. nov. is proposed. The type strain is BS-W13T ( = KCTC 42349T = CECT 8724T).
从韩国南海的潮滩中分离出一株革兰氏染色阴性、无运动性、需氧且多形态的细菌，命名为BS-W13T，并采用多相分类法研究其分类地位。菌株BS-W13T在25℃、pH 7.0 - 8.0以及1.0 - 2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法和最大简约法系统发育树表明，菌株BS-W13T与塞曼库海杆菌（Seohaeicola saemankumensis）模式菌株聚类，与该菌株的序列相似性最高（95.96%）。菌株BS-W13T与东海硫杆菌（Sulfitobacter donghicola）、紫菜硫杆菌（Sulfitobacter porphyrae）、地中海硫杆菌（Sulfitobacter mediterraneus）和滨海红杆菌（Roseobacter litoralis）模式菌株的16S rRNA基因序列相似性分别为95.95%、95.91%、95.72%和95.68%。菌株BS-W13T以Q-10作为主要泛醌，以C18 : 1ω7c作为主要脂肪酸。菌株BS-W13T的极性脂质谱以磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的氨基脂质和一种未鉴定的脂质为主要成分，与一些系统发育相关类群的不同。菌株BS-W13T的DNA G+C含量为58.1 mol%。系统发育数据以及化学分类和其他表型特征差异表明，菌株BS-W13T在α-变形菌纲红杆菌科中构成一个新属和新种，为此提出新属名为假塞曼库海杆菌属（Pseudoseohaeicola），新种名为潮间带假塞曼库海杆菌（Pseudoseohaeicola caenipelagi）。模式菌株为BS-W13T（= KCTC 42349T = CECT 8724T）。
