Park Sooyeon, Jung Yong-Taek, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2165-2171. doi: 10.1099/ijsem.0.001003. Epub 2016 Mar 4.
A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterial strain, designated DB-4T, was isolated from a tidal flat of the Yellow Sea, South Korea. Strain DB-4T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of approximately 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain DB-4T clustered with the type strain of Roseicyclus mahoneyensis, showing sequence similarity of 94.8 %. It exhibited sequence similarity values of 95.6-96.1 % to the type strains of Loktanella soesokkakensis, Loktanella hongkongensis, Loktanella variabilis, Loktanella aestuariicola and Loktanella cinnabarina. Strain DB-4T contained Q-10 as the predominant ubiquinone and C18:1ω7c and cyclo C19:0ω8c as the major fatty acids. The major polar lipids of strain DB-4T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The fatty acid and polar lipid profiles of strain DB-4T were distinguished from those of the type strains of R. mahoneyensis and some phylogenetically related Loktanella species. The DNA G+C content of strain DB-4T was 69.2 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain DB-4T is considered to represent a novel species of a new genus within the family Rhodobacteraceae, for which the name Pseudoroseicyclus aestuarii gen. nov., sp. nov. is proposed. The type strain ofPseudoroseicyclus aestuarii is DB-4T (=KCTC 52038T=CECT 9025T).
从韩国黄海潮滩分离出一株革兰氏阴性、无运动性、需氧的球状、卵形或杆状细菌菌株，命名为DB-4T。菌株DB-4T在30℃、pH 7.0 - 8.0以及约1.0 - 2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树显示，菌株DB-4T与马霍尼玫瑰环菌模式菌株聚类，序列相似性为94.8%。它与索索卡洛克坦氏菌、香港洛克坦氏菌、可变洛克坦氏菌、河口洛克坦氏菌和朱砂色洛克坦氏菌模式菌株的序列相似性值为95.6 - 96.1%。菌株DB-4T以Q-10作为主要泛醌，以C18:1ω7c和环C19:0ω8c作为主要脂肪酸。菌株DB-4T的主要极性脂为磷脂酰胆碱、磷脂酰甘油和一种未鉴定的氨基脂。菌株DB-4T的脂肪酸和极性脂谱与马霍尼玫瑰环菌模式菌株及一些系统发育相关的洛克坦氏菌属物种不同。菌株DB-4T的DNA G+C含量为69.2 mol%。基于系统发育、化学分类学和其他表型特征，菌株DB-4T被认为代表红杆菌科内一个新属的一个新物种，为此提出新属名河口假玫瑰环菌属（Pseudoroseicyclus），新种名河口假玫瑰环菌（Pseudoroseicyclus aestuarii）。河口假玫瑰环菌的模式菌株为DB-4T（=KCTC 52038T=CECT 9025T）。
