Park Sooyeon, Park Ji-Min, Choi Su Jung, Choi Jeehyun, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1344-1349. doi: 10.1099/ijsem.0.002677. Epub 2018 Mar 5.
A Gram-stain-negative, non-motile and ovoid or rod-shaped bacterial strain, DBTF-15, was isolated from a tidal flat sediment on the Yellow Sea in South Korea. Strain DBTF-15 grew optimally at 25-30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain DBTF-15 joined the cluster comprising the type strains of species of the genus Palleronia. Strain DBTF-15 exhibited higher 16S rRNA gene sequence similarity values to the type strains (96.5-96.7 %) of Maribius pelagius and Maribius salinus than to those (94.6-96.1 %) of the three species of the genus Palleronia. It exhibited 16S rRNA gene sequence similarity values of less than 93.9 % to the type strains of the other recognized species. Strain DBTF-15 contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain DBTF-15 were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain DBTF-15 was 68.7 mol%. The chemotaxonomic data and other differential phenotypic properties made it possible to distinguish strain DBTF-15 from the genera Maribius and Palleronia. On the basis of the data presented, strain DBTF-15 constitutes a novel genus and species within the class Alphaproteobacteria, for which the name Pseudomaribius aestuariivivens gen. nov., sp. nov. is proposed. The type strain is DBTF-15 (=KACC 19431=NBRC 113039).
从韩国黄海的潮滩沉积物中分离出一株革兰氏染色阴性、无运动性、卵形或杆状的细菌菌株DBTF-15。菌株DBTF-15在25-30°C、pH 7.0-8.0以及2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树显示，菌株DBTF-15加入了包含帕勒罗尼亚属物种模式菌株的簇。菌株DBTF-15与浮游马里比乌斯菌和盐生马里比乌斯菌的模式菌株的16S rRNA基因序列相似性值（96.5-96.7%）高于与帕勒罗尼亚属三个物种的模式菌株的相似性值（94.6-96.1%）。它与其他已确认物种的模式菌株的16S rRNA基因序列相似性值低于93.9%。菌株DBTF-15以Q-10作为主要泛醌，以C18:1ω7c作为主要脂肪酸。菌株DBTF-15的主要极性脂质是磷脂酰胆碱、磷脂酰甘油和一种未鉴定的氨基脂质。菌株DBTF-15的DNA G+C含量为68.7 mol%。化学分类数据和其他鉴别表型特征使得能够将菌株DBTF-15与马里比乌斯属和帕勒罗尼亚属区分开来。根据所提供的数据，菌株DBTF-15在α-变形菌纲内构成一个新的属和种，为此提出新属名假马里比乌斯菌属（Pseudomaribius）和新种名河口假马里比乌斯菌（Pseudomaribius aestuariivivens）。模式菌株为DBTF-15（=KACC 19431=NBRC 113039）。
