Pastorelli G, Roncarolo M G, Touraine J L, Rousset F, Pene J, de Vries J E
UNICET, Laboratory for Immunological Research, Dardilly, France.
Clin Exp Immunol. 1989 Dec;78(3):341-7.
Supernatants of both CD4+ and CD8+ alloreactive T cell clones induced IgM, IgG and IgA synthesis by peripheral blood lymphocytes (PBL) of healthy donors in vitro. These supernatants were also tested on their capacity to induce immunoglobulin production by PBL of four patients with CVI and one patient with CVI and thymoma. A low degree of IgM, IgG and IgA production was induced in one patient with CVI. In the patient with CVI and thymoma, induction of IgG and IgA synthesis was in the normal range, whereas IgM production was reduced. In the three other patients only a low production of IgM was induced. Interestingly, pre-incubation of the PBL for 24 h with interleukin-4 (IL-4) suppressed immunoglobulin production both by PBL of the patients with CVI and healthy donors. The strongest inhibitory effects were observed on IgA synthesis. These data indicate that B cells of three patients with CVI can not be induced to switch to IgG or IgA producing cells in vitro. In contrast, B cells of the patient with CVI and thymoma were able to respond to the relevant B cell growth and differentiation factors present in the T cell clone supernatants, suggesting that the T cells of this patient may fail to produce these factors. However, the proliferative responses of the T cells to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), were normal in all five patients tested. In addition, the interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production by PBL of the five patients was also in the normal range. Although only a small number of patients was tested, these results support the view that defects in both regulatory T cell functions and/or intrinsic B cell defects may contribute to the pathogenesis of CVI.
CD4+和CD8+同种异体反应性T细胞克隆的上清液在体外可诱导健康供体外周血淋巴细胞（PBL）合成IgM、IgG和IgA。还对这些上清液诱导4例常见变异型免疫缺陷（CVI）患者和1例CVI合并胸腺瘤患者的PBL产生免疫球蛋白的能力进行了检测。1例CVI患者诱导产生了低水平的IgM、IgG和IgA。在CVI合并胸腺瘤的患者中，IgG和IgA合成的诱导处于正常范围，而IgM产生减少。在其他3例患者中，仅诱导产生了低水平的IgM。有趣的是，将PBL与白细胞介素-4（IL-4）预孵育24小时可抑制CVI患者和健康供体的PBL产生免疫球蛋白。对IgA合成的抑制作用最强。这些数据表明，3例CVI患者的B细胞在体外不能被诱导转变为产生IgG或IgA的细胞。相反，CVI合并胸腺瘤患者的B细胞能够对T细胞克隆上清液中存在的相关B细胞生长和分化因子作出反应，这表明该患者的T细胞可能无法产生这些因子。然而，在所有检测的5例患者中，T细胞对植物血凝素（PHA）、刀豆蛋白A（Con A）和商陆有丝分裂原（PWM）的增殖反应均正常。此外，5例患者的PBL产生白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）也在正常范围内。尽管仅检测了少数患者，但这些结果支持以下观点，即调节性T细胞功能缺陷和/或内在B细胞缺陷可能参与了CVI的发病机制。
