Berntorp K, Eriksson K F, Berntorp E
Department of Medicine, University of Lund, Malmö General Hospital, Sweden.
Diabetes Res. 1989 Apr;10(4):171-4.
The transglutaminase involvement in phytohaemagglutinin (PHA) stimulated lymphocyte transformation (measured as (3H)thymidine incorporation) was studied in lymphocytes from 20 patients with type 2 diabetes mellitus and 20 healthy controls by including in the system the specific transglutaminase pseudosubstrate monodansylthiacadaverine (MDTC). In the presence of MDTC, (3H)thymidine incorporation was significantly and concentration-dependently reduced in both groups but more pronouncedly in the diabetes patients. The MDTC concentration needed to give a 50% reduction of the PHA-stimulated (3H)thymidine incorporation was significantly lower in lymphocytes from diabetic patients than in those from controls (p less than 0.02). The data suggest impaired lymphocyte transglutaminase function in type 2 diabetes mellitus.
通过在系统中加入特异性转谷氨酰胺酶假底物单丹磺酰硫代尸胺（MDTC），研究了转谷氨酰胺酶在植物血凝素（PHA）刺激的淋巴细胞转化（以（3H）胸腺嘧啶核苷掺入量衡量）中的作用，研究对象为20例2型糖尿病患者和20名健康对照者的淋巴细胞。在MDTC存在的情况下，两组的（3H）胸腺嘧啶核苷掺入量均显著且呈浓度依赖性降低，但在糖尿病患者中更为明显。使PHA刺激的（3H）胸腺嘧啶核苷掺入量降低50%所需的MDTC浓度，糖尿病患者淋巴细胞中的显著低于对照组（p<0.02）。数据表明2型糖尿病患者淋巴细胞转谷氨酰胺酶功能受损。
