School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin, 300162, China.
Adv Healthc Mater. 2015 Jun 3;4(8):1225-35. doi: 10.1002/adhm.201400817. Epub 2015 Mar 9.
Amphiphilic block copolymers containing biodegradable hydrophobic segments of depsipeptide based copolymers have been synthesized and explored as gene carriers for enhancing proliferation of endothelial cells in vitro. These polymers form nanoparticles (NPs) with positive charges on their surface, which could condense recombinant plasmids of enhanced green fluorescent protein plasmid and ZNF580 gene (pEGFP-ZNF580) and protect them against DNase I. ZNF580 gene is efficiently transported into EA.hy926 cells to promote their proliferation, whereby the transfection efficiency of NPs/pEGFP-ZNF580 is approximately similar to that of Lipofectamine 2000. These results indicate that the NPs might have potential as a carrier for pEGFP-ZNF580, which could support endothelialization of cardiovascular implants.
合成了含有基于多肽的可生物降解疏水性嵌段的两亲性嵌段共聚物，并将其作为基因载体用于体外增强内皮细胞的增殖。这些聚合物在其表面形成带正电荷的纳米颗粒（NPs），可使重组增强型绿色荧光蛋白质粒和 ZNF580 基因（pEGFP-ZNF580）的质粒凝聚，并保护其免受 DNA 酶 I 的影响。ZNF580 基因有效地转染 EA.hy926 细胞，促进其增殖，其中 NPs/pEGFP-ZNF580 的转染效率与 Lipofectamine 2000 大致相似。这些结果表明，NPs 可能作为 pEGFP-ZNF580 的载体具有潜力，可支持心血管植入物的内皮化。
