Feng Yakai, Liu Wen, Ren Xiangkui, Lu Wei, Guo Mengyang, Behl Marc, Lendlein Andreas, Zhang Wencheng
School of Chemical Engineering and Technology, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin University, Tianjin 300072, China.
Tianjin University⁻Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin 300072, China.
Polymers (Basel). 2016 Feb 23;8(3):58. doi: 10.3390/polym8030058.
Functional artificial vascular meshes from biodegradable polymers have been widely explored for certain tissue engineered meshes. Still, the foreign body reaction and limitation in endothelialization are challenges for such devices. Here, degradable meshes from phase-segregated multiblock copolymers consisting of poly(ε-caprolactone) (PCL) and polydepsipeptide segments are successfully prepared by electrospinning and electrospraying techniques. The pEGFP-ZNF580 plasmid microparticles (MPs-pZNF580) were loaded into the electrospun meshes to enhance endothelialization. These functional meshes were evaluated and . The adhesion and proliferation of endothelial cells on the meshes were enhanced in loaded mesh groups. Moreover, the hemocompatibility and the tissue response of the meshes were further tested. The complete tests showed that the vascular meshes modified with MPs-pZNF580 possessed satisfactory performance with an average fiber diameter of 550 ± 160 nm, tensile strength of 27 ± 3 MPa, Young's modulus of 1. 9 ± 0.2 MPa, water contact angle of 95° ± 2°, relative cell number of 122% ± 1% after 7 days of culture, and low blood platelet adhesion as well as weak inflammatory reactions compared to control groups.
用于某些组织工程网片的可生物降解聚合物功能性人工血管网片已得到广泛研究。然而，异物反应和内皮化的局限性仍是此类装置面临的挑战。在此，通过静电纺丝和电喷雾技术成功制备了由聚（ε-己内酯）（PCL）和聚（酯肽）链段组成的相分离多嵌段共聚物可降解网片。将pEGFP-ZNF580质粒微粒（MPs-pZNF580）加载到静电纺网片中以增强内皮化。对这些功能性网片进行了评估。加载网片组中网片上内皮细胞的黏附和增殖得到增强。此外，还进一步测试了网片的血液相容性和组织反应。完整测试表明，用MPs-pZNF580修饰的血管网片性能令人满意，平均纤维直径为550±160nm，拉伸强度为27±3MPa，杨氏模量为1.9±0.2MPa，水接触角为95°±2°，培养7天后相对细胞数为122%±1%，与对照组相比血小板黏附率低且炎症反应弱。
