Kumar Rajesh, Pahal Vikas, Singh Jasbir
Department of Biochemistry, Kurukshetra University, Kurukshetra - 136119, Haryana, India.
J Clin Exp Hepatol. 2011 Sep;1(2):73-6. doi: 10.1016/S0973-6883(11)60125-4. Epub 2011 Nov 9.
Hepatitis B virus (HBV) isolates (21) from Punjab (North India) were studied for genotype distribution and precore/core promoter mutations. Assays of alanine aminotransaminase (ALT) and HBeAg were performed in all isolates. Genotypes were determined in all the samples by restriction fragment length polymorphism and the precore/core promoter mutations were studied by amplification and by direct sequencing of precore/core promoter region. Sixty-two percent of the isolates had higher ALT levels and 57% of the isolates were HBeAg negative. It was observed that 90% of the isolates were HBV D genotype (subgenotype D1 and D2) and 10% of the isolates were HBV A genotype (subgenotype A1). Amplification and sequencing of the precore/core promoter region showed 1762(A-T) and 1764(G-A) mutations in 29% and 19% of the isolates, respectively. 1809(C/T) mutation was observed in 71% of the isolates under study. Novel precore and core promoter mutations like 1690(A), 1695(A/T/G), 1700(A/C), 1703(c), 1850(A) and 1915(A/G) were observed in HBV-infected population of the state of Punjab (North India). Deletion and insertional mutations were also observed in some patients. (J CLIN EXP HEPATOL 2011;1:73-76).
对来自旁遮普邦（印度北部）的21株乙型肝炎病毒（HBV）分离株进行了基因型分布和前核心/核心启动子突变研究。对所有分离株进行了丙氨酸氨基转移酶（ALT）和HBeAg检测。通过限制性片段长度多态性确定所有样本的基因型，并通过扩增和前核心/核心启动子区域的直接测序研究前核心/核心启动子突变。62%的分离株ALT水平较高，57%的分离株HBeAg阴性。观察到90%的分离株为HBV D基因型（D1和D2亚基因型），10%的分离株为HBV A基因型（A1亚基因型）。前核心/核心启动子区域的扩增和测序显示，分别有29%和19%的分离株存在1762（A-T）和1764（G-A）突变。在71%的研究分离株中观察到1809（C/T）突变。在旁遮普邦（印度北部）的HBV感染人群中观察到了新的前核心和核心启动子突变，如1690（A）、1695（A/T/G）、1700（A/C）、1703（c）、1850（A）和1915（A/G）。在一些患者中还观察到了缺失和插入突变。（《临床与实验肝脏病学杂志》2011年；1：73 - 76）
