Asaad Ahmed Morad, Al-Ayed Mohamed Saeed Zayed, Aleraky Mohamed, Qureshi Mohamed Ansar
Department of Microbiology, College of Medicine, Najran University, Najran, Saudi Arabia.
College of Applied Medical Sciences, Department of Pediatric, College of Medicine, Najran University, Najran, Saudi Arabia.
Braz J Infect Dis. 2015 Sep-Oct;19(5):525-8. doi: 10.1016/j.bjid.2015.03.007. Epub 2015 May 18.
The distribution of hepatitis B virus genotypes in Saudi Arabia is largely unknown. To the best of our knowledge there are no data available about HBV genotypes in southwestern region of the country. This study aimed to determine the epidemiologic distribution of hepatitis B virus genotypes in chronic hepatitis B patients in southwestern region, and to verify possible correlations between these genotypes and the clinical symptoms. A total of 160 patients with chronic hepatitis B infection were enrolled in this study. Sera were tested for liver function tests, hepatitis B virus markers and DNA load by standard procedures. HBV genotyping was performed by 2-tube nested PCR for determination of six genotypes (A-F). Genotype D was the most common, found in 135 (84.4%) patients, followed by A (18; 11.3%) and E (7; 4.3%). The rate of HBeAg positivity in genotype D patients was significantly lower compared with that in genotype A and E patients (p=0.01). There was no significant association between HBV genotypes and age, gender, liver function tests, or HBV DNA load. Genotypes D and E were predominant in chronic hepatitis B patients in southwestern Saudi Arabia. Awareness of hepatitis B virus serologic and genotypic patterns might help in the formulation of management plans, predicting clinical outcomes and updating prevention strategies.
沙特阿拉伯乙型肝炎病毒基因型的分布情况很大程度上尚不清楚。据我们所知，该国西南部地区尚无关于乙肝病毒基因型的数据。本研究旨在确定沙特阿拉伯西南部地区慢性乙型肝炎患者中乙肝病毒基因型的流行病学分布，并验证这些基因型与临床症状之间可能存在的相关性。本研究共纳入了160例慢性乙型肝炎感染患者。通过标准程序对血清进行肝功能检查、乙肝病毒标志物检测及DNA载量检测。采用两管巢式PCR进行HBV基因分型，以确定六种基因型（A - F）。D基因型最为常见，在135例（84.4%）患者中发现，其次是A基因型（18例；11.3%）和E基因型（7例；4.3%）。D基因型患者的HBeAg阳性率显著低于A基因型和E基因型患者（p = 0.01）。HBV基因型与年龄、性别、肝功能检查或HBV DNA载量之间无显著关联。D基因型和E基因型在沙特阿拉伯西南部慢性乙型肝炎患者中占主导地位。了解乙肝病毒的血清学和基因型模式可能有助于制定管理计划、预测临床结果以及更新预防策略。
