Zhang Junbo, He Xue, Ma Yueying, Liu Yanli, Shi Huaiyin, Guo Weiwei, Liu Liangfa
Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital Beijing 100034, China.
Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing 100853, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):244-51. eCollection 2015.
Rho-associated coiled-coil containing protein kinase (ROCK) over-expression has been implicated in the progression of many tumor types. The aim of this study was to explore the roles of ROCK1 and ROCK2 in human laryngeal squamous cell carcinoma (LSCC). ROCK1 and ROCK2 expression levels were examined in 50 cases of human LSCC samples by immunohistochemistry. Effects of ROCK1 and ROCK2 on LSCC cell proliferation and motility were investigated in the presence of the ROCK inhibitor Y-27632. The results showed that ROCK1 expression was positively correlated with tumor size and lymph node metastasis (P < 0.05); ROCK2 positively correlated with tumor size (P < 0.05). Inhibition of ROCK1 and ROCK2 by Y-27632 significantly inhibits proliferation, migration, and invasion of LSCC cells. Our data indicate that expression of ROCK1 and ROCK2 are closely associated with tumor growth and lymph node metastasis of LSCC. Thus, these two ROCK isoforms may be useful as molecular makers for LSCC diagnosis and may be useful therapeutic targets as well.
Rho相关卷曲螺旋蛋白激酶（ROCK）的过表达与多种肿瘤类型的进展有关。本研究的目的是探讨ROCK1和ROCK2在人喉鳞状细胞癌（LSCC）中的作用。采用免疫组织化学方法检测50例人LSCC样本中ROCK1和ROCK2的表达水平。在存在ROCK抑制剂Y-27632的情况下，研究ROCK1和ROCK2对LSCC细胞增殖和运动的影响。结果显示，ROCK1表达与肿瘤大小和淋巴结转移呈正相关（P < 0.05）；ROCK2与肿瘤大小呈正相关（P < 0.05）。Y-27632抑制ROCK1和ROCK2可显著抑制LSCC细胞的增殖、迁移和侵袭。我们的数据表明，ROCK1和ROCK2的表达与LSCC的肿瘤生长和淋巴结转移密切相关。因此，这两种ROCK异构体可能作为LSCC诊断的分子标志物，也可能是有用的治疗靶点。
