Ma Jun, Liu Shaofeng, Zhang Wei, Zhang Fan, Wang Sufen, Wu Lifeng, Yan Renchun, Wu Lijuan, Wang Chuanxi, Zha Zhihong, Sun Jingwu
Department of Otolaryngology-Head and Neck Surgery, Anhui Medical University Affiliated Anhui Provincial Hospital, Hefei, China.
Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
Pathology. 2016 Dec;48(7):691-696. doi: 10.1016/j.pathol.2016.08.005. Epub 2016 Oct 22.
N-myc downstream-regulated gene 3 (NDRG3), which belongs to the NDRG family, is believed to play important roles in human cancer. In this present study, one-step quantitative reverse transcription-polymerase chain reaction (qPCR) and western blotting tests with 10 fresh-frozen laryngeal squamous cell carcinoma (LSCC) samples and immunohistochemistry (IHC) analysis in 109 LSCC cases were performed to investigate the relationship between NDRG3 expression and the clinicopathological characteristics of LSCC. Results demonstrated that NDRG3 mRNA and protein expression levels were statistically higher in LSCC tissues than that in non-cancerous tissues (all p<0.05). IHC data showed that the NDRG3 protein expression was remarkably correlated with lymph node metastasis (p=0.043). Univariate and multivariate survival analysis implied that high NDRG3 expression (p=0.004), lymph node metastasis (p=0.044) and TNM stage (p=0.020) were independently associated with the unfavourable overall survival of patients with LSCC. The above findings suggested that NDRG3 may be identified as a novel biomarker predicting the prognosis of LSCC.
N-myc下游调控基因3（NDRG3）属于NDRG家族，被认为在人类癌症中发挥重要作用。在本研究中，对10份新鲜冷冻的喉鳞状细胞癌（LSCC）样本进行了一步法定量逆转录-聚合酶链反应（qPCR）和蛋白质印迹检测，并对109例LSCC病例进行了免疫组织化学（IHC）分析，以研究NDRG3表达与LSCC临床病理特征之间的关系。结果表明，LSCC组织中NDRG3 mRNA和蛋白表达水平在统计学上高于非癌组织（所有p<0.05）。免疫组化数据显示，NDRG3蛋白表达与淋巴结转移显著相关（p=0.043）。单因素和多因素生存分析表明，NDRG3高表达（p=0.004）、淋巴结转移（p=0.044）和TNM分期（p=0.020）与LSCC患者不良的总生存期独立相关。上述发现表明，NDRG3可能被鉴定为预测LSCC预后的一种新型生物标志物。
