Cen Zhihong, Guo Yujie, Kong Qing, Zhou Qiuxi, Wu Weifeng
Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):830-5. eCollection 2015.
Interleukin-10 (IL-10)-producing B cells, a subset of regulatory B cells, play critical roles in autoimmune and infectious diseases. However, the role of IL-10-producing B cells in acute viral myocarditis (AVMC) remains unknown.
BALB/c mice were intraperitoneally (i. p.) infected with coxsackievirus B3 (CVB3) to establish AVMC models (AVMC group), while control mice (control group) were treated with phosphate-buffered saline (PBS) i. p. According to the time after injection, the AVMC group mice or control group mice were randomly separated into 1 week and 2 week subgroup. Myocardial histopathological changes were observed by hematoxylin and eosin staining and the frequency of splenic IL-10-producing B cells was measured by flow cytometry.
Histopathologic examination of heart tissues showed that mice infected with CVB3 developed AVMC. Compared with control group, the frequency of splenic IL-10-producing B cells was increased significantly in the AVMC group, with the 1 week AVMC subgroup (3.58 ± 0.47%) higher than the 2 week AVMC subgroup (2.50 ± 0.42%) (all P < 0.05).
IL-10-producing B cells are increased in CVB3-induced AVMC, indicating that IL-10-producing B cells may play an important role in the pathogenesis of CVB3-induced AVMC.
产生白细胞介素-10（IL-10）的B细胞是调节性B细胞的一个亚群，在自身免疫性疾病和感染性疾病中发挥关键作用。然而，产生IL-10的B细胞在急性病毒性心肌炎（AVMC）中的作用尚不清楚。
将BALB/c小鼠腹腔注射柯萨奇病毒B3（CVB3）以建立AVMC模型（AVMC组），而对照小鼠（对照组）腹腔注射磷酸盐缓冲盐水（PBS）。根据注射后的时间，将AVMC组小鼠或对照组小鼠随机分为1周和2周亚组。通过苏木精和伊红染色观察心肌组织病理学变化，并用流式细胞术检测脾脏中产生IL-10的B细胞频率。
心脏组织的组织病理学检查显示，感染CVB3的小鼠发生了AVMC。与对照组相比，AVMC组脾脏中产生IL-10的B细胞频率显著增加，1周AVMC亚组（3.58±0.47%）高于2周AVMC亚组（2.50±0.42%）（均P<0.05）。
在CVB3诱导的AVMC中，产生IL-10的B细胞增加，表明产生IL-10的B细胞可能在CVB3诱导的AVMC发病机制中起重要作用。
