产生白细胞介素-10的B细胞参与柯萨奇病毒B3诱导的急性病毒性心肌炎的发病机制。
IL-10-producing B cells involved in the pathogenesis of Coxsackie virus B3-induced acute viral myocarditis.
作者信息
Cen Zhihong, Guo Yujie, Kong Qing, Zhou Qiuxi, Wu Weifeng
机构信息
Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
出版信息
Int J Clin Exp Pathol. 2015 Jan 1;8(1):830-5. eCollection 2015.
BACKGROUND
Interleukin-10 (IL-10)-producing B cells, a subset of regulatory B cells, play critical roles in autoimmune and infectious diseases. However, the role of IL-10-producing B cells in acute viral myocarditis (AVMC) remains unknown.
METHODS
BALB/c mice were intraperitoneally (i. p.) infected with coxsackievirus B3 (CVB3) to establish AVMC models (AVMC group), while control mice (control group) were treated with phosphate-buffered saline (PBS) i. p. According to the time after injection, the AVMC group mice or control group mice were randomly separated into 1 week and 2 week subgroup. Myocardial histopathological changes were observed by hematoxylin and eosin staining and the frequency of splenic IL-10-producing B cells was measured by flow cytometry.
RESULTS
Histopathologic examination of heart tissues showed that mice infected with CVB3 developed AVMC. Compared with control group, the frequency of splenic IL-10-producing B cells was increased significantly in the AVMC group, with the 1 week AVMC subgroup (3.58 ± 0.47%) higher than the 2 week AVMC subgroup (2.50 ± 0.42%) (all P < 0.05).
CONCLUSIONS
IL-10-producing B cells are increased in CVB3-induced AVMC, indicating that IL-10-producing B cells may play an important role in the pathogenesis of CVB3-induced AVMC.
背景
产生白细胞介素-10（IL-10）的B细胞是调节性B细胞的一个亚群，在自身免疫性疾病和感染性疾病中发挥关键作用。然而，产生IL-10的B细胞在急性病毒性心肌炎（AVMC）中的作用尚不清楚。
方法
将BALB/c小鼠腹腔注射柯萨奇病毒B3（CVB3）以建立AVMC模型（AVMC组），而对照小鼠（对照组）腹腔注射磷酸盐缓冲盐水（PBS）。根据注射后的时间，将AVMC组小鼠或对照组小鼠随机分为1周和2周亚组。通过苏木精和伊红染色观察心肌组织病理学变化，并用流式细胞术检测脾脏中产生IL-10的B细胞频率。
结果
心脏组织的组织病理学检查显示，感染CVB3的小鼠发生了AVMC。与对照组相比，AVMC组脾脏中产生IL-10的B细胞频率显著增加，1周AVMC亚组（3.58±0.47%）高于2周AVMC亚组（2.50±0.42%）（均P<0.05）。
结论
在CVB3诱导的AVMC中，产生IL-10的B细胞增加，表明产生IL-10的B细胞可能在CVB3诱导的AVMC发病机制中起重要作用。
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