Huang Ying, Ogutu James O, Gu Jiarui, Ding Fengshu, You Yuhong, Huo Yan, Zhao Hong, Li Wenjing, Zhang Zhiwei, Zhang Wenli, Chen Xiaobei, Fu Yingmei, Zhang Fengmin
Department of Microbiology, Pathogenic Biology, Harbin Medical University, Harbin, Heilongjiang 150081, China ; Heilongjiang Provincial Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, China.
Department of Microbiology, Pathogenic Biology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Biomed Res Int. 2015;2015:168292. doi: 10.1155/2015/168292. Epub 2015 Feb 10.
The objective of this study was to compare quinolone resistance and gyrA mutations in clinical isolates of Klebsiella pneumoniae and Escherichia coli from Chinese adults who used quinolone in the preceding month and children without any known history of quinolone administration. The antimicrobial susceptibilities of 61 isolates from children and 79 isolates from adults were determined. The mutations in the quinolone resistance-determining regions in gyrA gene were detected by PCR and DNA sequencing. Fluoroquinolone resistance and types of gyrA mutations in isolates from children and adults were compared and statistically analyzed. No significant differences were detected in the resistance rates of ciprofloxacin and levofloxacin between children and adults among isolates of the two species (all P > 0.05). The double mutation Ser83→Leu + Asp87→Asn in the ciprofloxacin-resistant isolates occurred in 73.7% isolates from the children and 67.9% from the adults, respectively (P = 0.5444). Children with no known history of quinolone administration were found to carry fluoroquinolone-resistant Enterobacteriaceae isolates. The occurrence of ciprofloxacin resistance and the major types of gyrA mutations in the isolates from the children were similar to those from adults. The results indicate that precautions should be taken on environmental issues resulting from widespread transmission of quinolone resistance.
本研究的目的是比较肺炎克雷伯菌和大肠杆菌临床分离株中的喹诺酮耐药性及gyrA基因突变情况，这些分离株来自前一个月使用过喹诺酮的中国成年人以及无任何喹诺酮用药史的儿童。测定了61株儿童分离株和79株成人分离株的抗菌药敏性。通过聚合酶链反应（PCR）和DNA测序检测gyrA基因喹诺酮耐药决定区的突变。比较并统计分析了儿童和成人分离株中的氟喹诺酮耐药性及gyrA基因突变类型。在这两个菌种的分离株中，儿童和成人对环丙沙星和左氧氟沙星的耐药率未检测到显著差异（所有P>0.05）。环丙沙星耐药分离株中的双重突变Ser83→Leu + Asp87→Asn分别出现在73.7%的儿童分离株和67.9%的成人分离株中（P = 0.5444）。发现无喹诺酮用药史的儿童携带耐氟喹诺酮的肠杆菌科分离株。儿童分离株中环丙沙星耐药性的发生情况及gyrA基因突变的主要类型与成人相似。结果表明，应针对喹诺酮耐药性广泛传播所导致的环境问题采取预防措施。
