University of Veterinary Medicine Hannover, Hannover, Germany.
Emerg Infect Dis. 2013 Aug;19(8):1253-9. doi: 10.3201/eid1908.120879.
During 2010, we evaluated the presence of extended-spectrum β-lactamase- and AmpC-producing enterobacteria in broiler chickens at slaughter. Samples (70 carcasses and 51 ceca) from 4 flocks were analyzed by direct plating and after enrichment. Extended-spectrum β-lactamase producers were found in 88.6% and 72.5% of carcasses and ceca, respectively; AmpC producers were found in 52.9% and 56.9% of carcasses and ceca, respectively. Most isolates were identified as Escherichia coli; Enterobacter cloacae (cecum) and Proteus mirabilis (carcass) were found in 2 samples each. Molecular characterization revealed the domination of CTX-M genes; plasmidic AmpC was CIT-like. Phylogenetic grouping of E. coli showed types A (31.5%), B1 (20.2%), B2 (13.5%), and D (34.8%). These findings provide evidence that healthy broilers in Germany are a source for the dissemination of transmissible resistance mechanisms in enterobacteria brought from the rearing environment into the food chain during slaughtering.
2010 年，我们评估了在屠宰时肉鸡中携带广谱β-内酰胺酶和 AmpC 型产酶肠杆菌的情况。对 4 个鸡群的 70 个胴体和 51 个盲肠样本进行了直接平板培养和富集后分析。在胴体和盲肠中分别发现了 88.6%和 72.5%的产广谱β-内酰胺酶的菌株；在胴体和盲肠中分别发现了 52.9%和 56.9%的产 AmpC 酶的菌株。大多数分离株被鉴定为大肠杆菌；2 个样本中分别发现了阴沟肠杆菌（盲肠）和奇异变形杆菌（胴体）。分子特征表明 CTX-M 基因占主导地位；质粒型 AmpC 是 CIT 样的。大肠杆菌的系统发育分组显示 A 型（31.5%）、B1 型（20.2%）、B2 型（13.5%）和 D 型（34.8%）。这些发现表明，来自饲养环境的可传播耐药机制在进入食物链的过程中，通过德国健康肉鸡在屠宰时被传播。
