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恶性胸膜间皮瘤和良性石棉相关胸腔积液的微小RNA和信使核糖核酸特征

MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion.

作者信息

Ak Guntulu, Tomaszek Sandra C, Kosari Farhad, Metintas Muzaffer, Jett James R, Metintas Selma, Yildirim Huseyin, Dundar Emine, Dong Jie, Aubry Marie Christine, Wigle Dennis A, Thomas Charles F

机构信息

Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.

Division of Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA.

出版信息

Biomed Res Int. 2015;2015:635748. doi: 10.1155/2015/635748. Epub 2015 Feb 1.

DOI:10.1155/2015/635748
PMID:25756049
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4331157/
Abstract

INTRODUCTION

We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion.

METHODS

Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols.

RESULTS

We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway.

CONCLUSIONS

Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.

摘要

引言

我们研究了恶性胸膜间皮瘤患者或良性石棉相关胸腔积液患者胸膜组织中微小RNA（miRNA）和信使核糖核酸（mRNA）的表达情况。

方法

对总共18例恶性胸膜间皮瘤患者和6例良性石棉相关胸腔积液患者的新鲜冷冻组织进行了研究。使用标准方案进行mRNA和miRNA的表达谱分析。

结果

我们发现与良性石棉相关胸腔积液相比，恶性胸膜间皮瘤中有多种miRNA显著上调。人miR-484、人miR-320、人let-7a和人miR-125a-5p能够区分恶性和良性疾病。还鉴定出了动态调节的mRNA。与良性石棉相关胸腔积液相比，MET是恶性胸膜间皮瘤中表达最高的基因。对miRNA-mRNA相互作用的综合分析表明Notch信号通路内有多个改变的靶点。

结论

特定的miRNA和mRNA可能在区分恶性胸膜间皮瘤患者与良性石棉相关胸腔积液方面具有诊断价值。这些研究对于居住在间皮瘤高发地区的患者可能特别有帮助。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/b241df322550/BMRI2015-635748.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/6b4e85c3a451/BMRI2015-635748.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/5c8fc6ea34ba/BMRI2015-635748.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/b241df322550/BMRI2015-635748.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/6b4e85c3a451/BMRI2015-635748.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/5c8fc6ea34ba/BMRI2015-635748.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b851/4331157/b241df322550/BMRI2015-635748.003.jpg

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