Woodall Daniel W, Wang Beixi, Inutan Ellen D, Narayan Srinivas B, Trimpin Sarah
†Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, United States.
‡Detroit Medical Center: Detroit Hospital (DMC), Detroit, Michigan 48201, United States.
Anal Chem. 2015;87(9):4667-74. doi: 10.1021/ac504475x. Epub 2015 Apr 17.
Matrix assisted ionization vacuum (MAIV) rapidly generates gas-phase analyte ions from subliming solid-phase matrix:analyte crystals for analysis by mass spectrometry (MS). Ionization from the solid-phase allows the use of a variety of surfaces for introducing matrix:analyte samples to the vacuum of a mass spectrometer, including common laboratory materials, such as disposable pipet tips, filter paper, tooth picks, and nylon mesh. MAIV is shown here to be capable of analyses as fast as 3 s per sample with achievable sensitivities in the low femtomole range. MAIV-MS coupled with ion mobility spectrometry (IMS)-MS and tandem mass spectrometry (MS/MS) is shown to be especially powerful for analysis and characterization of a wide range of molecules ranging from small molecules such as drugs and metabolites (∼300 Da) to intact proteins (25.6 kDa). Automated sample introduction is demonstrated on two different commercial mass spectrometers using a programmable XYZ stage. A MAIV high-throughput nontargeted MS(E) approach is also demonstrated utilizing IMS for rapid characterization of small molecules and peptides from standard solutions, as well as drug spiked human urine.
基质辅助电离真空（MAIV）能从升华的固相基质：分析物晶体中快速产生气相分析物离子，用于质谱（MS）分析。固相电离允许使用各种表面将基质：分析物样品引入质谱仪的真空环境，包括常见的实验室材料，如一次性移液器吸头、滤纸、牙签和尼龙网。本文表明MAIV能够以每个样品3秒的速度进行分析，灵敏度可达低飞摩尔范围。MAIV-MS与离子淌度光谱（IMS）-MS和串联质谱（MS/MS）联用，对于从药物和代谢物等小分子（约300 Da）到完整蛋白质（25.6 kDa）等广泛分子的分析和表征特别有效。使用可编程XYZ平台在两种不同的商用质谱仪上展示了自动进样。还展示了一种MAIV高通量非靶向MS(E)方法，利用IMS对标准溶液以及加标药物的人尿液中的小分子和肽进行快速表征。
