Wildes Andrew, Khadeeva Liya, Trewby William, Valle-Orero Jessica, Studer Andrew, Garden Jean-Luc, Peyrard Michel
†Institut Laue-Langevin, CS 20156, 71 Avenue des Martyrs, 38042 Grenoble, France.
‡Institut de Physique de Rennes, UMR UR1 - CNRS 6251, 35042 Rennes Cedex, France.
J Phys Chem B. 2015 Mar 26;119(12):4441-9. doi: 10.1021/acs.jpcb.5b01343. Epub 2015 Mar 18.
A pilot study of the possibility to investigate temperature-dependent neutron scattering from fiber-DNA in solution is presented. The study aims to establish the feasibility of experiments to probe the influence of spatial confinement on the structural correlation and the formation of denatured bubbles in DNA during the melting transition. Calorimetry and neutron scattering experiments on fiber samples immersed in solutions of poly(ethylene glycol) (PEG) prove that the melting transition occurs in these samples, that the transition is reversible to some degree, and that the transition is broader in temperature than for humidified fiber samples. The PEG solutions apply an osmotic pressure that maintains the fiber orientation, establishing the feasibility of future scattering experiments to study the melting transition in these samples.
本文介绍了一项关于研究溶液中纤维-DNA温度依赖性中子散射可能性的初步研究。该研究旨在确定实验的可行性，以探究空间限制对DNA在熔解转变过程中结构相关性和变性气泡形成的影响。对浸入聚乙二醇（PEG）溶液中的纤维样品进行的量热法和中子散射实验证明，这些样品中发生了熔解转变，该转变在一定程度上是可逆的，并且该转变在温度上比加湿纤维样品更宽。PEG溶液施加渗透压以维持纤维取向，从而确定了未来散射实验研究这些样品中熔解转变的可行性。
