Institut Laue-Langevin, Grenoble, France.
ICCRAM, University of Burgos, Burgos, Spain.
Biopolymers. 2021 Mar;112(3):e23422. doi: 10.1002/bip.23422. Epub 2021 Feb 18.
The melting transition of Li-DNA fibers immersed in ethanol-water solutions has been studied using calorimetry and neutron diffraction techniques. The data have been analyzed using the Peyrard-Bishop-Dauxois model to determine the strengths of the intra- and inter-base pair potentials. The data and analysis show that the potentials are weaker than those for DNA in water. They become weaker still and the DNA less stable as the ethanol concentration increases but, conversely, the fibers become more compact and the distances between base pairs become more regular. The results show that the melting transition is relatively insensitive to local confinement and depends more on the interaction between the DNA and its aqueous environment.
使用量热法和中子衍射技术研究了浸入乙醇-水溶液中的 Li-DNA 纤维的熔融转变。使用 Peyrard-Bishop-Dauxois 模型对数据进行了分析，以确定碱基对内和碱基对间的势能强度。数据和分析表明，这些势能比 DNA 在水中的弱。随着乙醇浓度的增加，势能变得更弱，DNA 变得越不稳定，但相反，纤维变得更紧凑，碱基对之间的距离变得更规则。结果表明，熔融转变对局部限制相对不敏感，而更多地取决于 DNA 与其水环境之间的相互作用。
