Choromanska Anna, Kulbacka Julita, Rembialkowska Nina, Pilat Justyna, Drag-Zalesinska Małgorzata, Wysocka Teresa, Garbiec Arnold, Kotulska Malgorzata, Saczko Jolanta
aDepartment of Medical Biochemistry bDepartment of Histology and Embryology, Wroclaw Medical University cDepartment of Animal Developmental Biology, University of Wroclaw dFaculty of Fundamental Problems of Technology, Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wroclaw, Poland.
Melanoma Res. 2015 Jun;25(3):210-24. doi: 10.1097/CMR.0000000000000153.
Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.
由于黑色素瘤中黑色素的吸收与光敏剂在光敏剂激发光波长下的吸收之间存在竞争，光动力疗法一直被认为对黑色素瘤无效。黑色素瘤表现出相当大的异质性和对光疗的抗性。通过电穿孔可增强光敏剂通过膜上瞬时孔隙的转运，从而增强光动力疗法的有效性。在本研究中，光动力疗法联合电穿孔在体外对人黑色素瘤细胞系黑素性黑色素瘤（MeWo）和无黑素性黑色素瘤（C32）进行了测试。对人角质形成细胞（HaCaT）进行了对照实验。使用卟吩姆钠作为光敏剂。进行了光敏剂分布、克隆效率测试、彗星试验以及凋亡蛋白评估。在光动力疗法前后测定了黑色素水平。实验表明电穿孔有效地支持了光动力方法。发现光动力疗法联合电穿孔能有效诱导黑素性和无黑素性黑色素瘤细胞凋亡。
