骨膜蛋白在牙髓细胞中的表达。

The expression of periostin in dental pulp cells.

作者信息

Wiesen Robert M, Padial-Molina Miguel, Volk Sarah L, McDonald Neville, Chiego Daniel, Botero Tatiana, Rios Hector F

机构信息

Resident of Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA.

Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA; Department of Oral Surgery and Implant Dentistry, School of Dentistry, University of Granada, Granada, Spain.

出版信息

Arch Oral Biol. 2015 May;60(5):760-7. doi: 10.1016/j.archoralbio.2015.02.008. Epub 2015 Feb 21.

DOI:10.1016/j.archoralbio.2015.02.008

PMID:25757150

Abstract

BACKGROUND AND OBJECTIVE

Dental pulp repair is a common process triggered by microbial and mechanical challenges. Matricellular modulators, such as periostin, are key for extracellular matrix stability and tissue healing. In the scope of the dental pulp, periostin expression has been reported during development and active dentinogenesis. However, the specific dental pulp cell population capable of expressing periostin in response to known regulators has not been clearly defined. Among the different relevant cell populations (i.e., stem cells, fibroblasts and pre-odontoblasts) potentially responsible for periostin expression in the dental pulp, this study aimed to determine which is the primary responder to periostin regulators.

METHODS

Human dental pulp stem cells (DPSCs), human dental pulp fibroblasts (DPFs), and rat odontoblast-like cells (MDPC-23) were treated with different concentrations of TGF-β1 or different regimens of biomechanical stimulation to evaluate periostin expression by qRT-PCR, Western blot and ELISA. Statistical analyses were performed by Student's t-test and ANOVA with Fisher's LSD post hoc tests (p ≤ 0.05).

RESULTS

DPSC and MDPC-23 showed a statistically significant increase in periostin mRNA expression after exposure to TGF-β1 for 48 h. TGF-β1 also up-regulated periostin protein levels in DPSC. However, periostin significantly down-regulated protein expression in DPF. Different regimens of biomechanical stimulation showed different patterns in protein and mRNA periostin expression.

CONCLUSIONS

Expression of periostin was identified in each of the analysed dental pulp cell lines, which can be regulated by TGF-β1 and biomechanical stimulation. Overall, DPSCs are the most responsive cells to stimulation.

摘要

背景与目的

牙髓修复是由微生物和机械刺激引发的常见过程。诸如骨膜蛋白等基质细胞调节剂对于细胞外基质稳定性和组织愈合至关重要。在牙髓范围内，已报道在发育和活跃的牙本质形成过程中存在骨膜蛋白表达。然而，能够响应已知调节因子而表达骨膜蛋白的特定牙髓细胞群尚未明确界定。在牙髓中可能负责骨膜蛋白表达的不同相关细胞群（即干细胞、成纤维细胞和前成牙本质细胞）中，本研究旨在确定哪一种是对骨膜蛋白调节因子的主要应答者。

方法

用不同浓度的转化生长因子-β1（TGF-β1）或不同方案的生物力学刺激处理人牙髓干细胞（DPSCs）、人牙髓成纤维细胞（DPFs）和大鼠成牙本质样细胞（MDPC-23），通过实时定量逆转录聚合酶链反应（qRT-PCR）、蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）评估骨膜蛋白表达。采用学生t检验和方差分析以及Fisher最小显著差异法进行统计学分析（p≤0.05）。