Mothersill Carmel, Austin Dawn, Fernandez-Palomo Cristian, Seymour Colin, Auchinachie Niall, Austin Brian
Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada
School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.
FEMS Microbiol Lett. 2015 Mar;362(5). doi: 10.1093/femsle/fnu058. Epub 2014 Dec 12.
Rainbow trout (Oncorhynchus mykiss, Walbaum) were challenged intraperitoneally with a sublethal dose of Vibrio anguillarum VIB1 and allowed to recover. Then, after 7 days, naïve fish, (designated as 'bystander' fish) which had never been exposed to the pathogen, were introduced to the same tank. These swam with the adapted (recovered) fish for 7 days before both groups and a control (never exposed directly to the pathogen or to recovered fish) group were exposed to a lethal dose of VIB1. Mortality records were 100% in the control group within 3 days, 47% in the adapted group and 60% in the unchallenged bystander group, which swam with the adapted group. In both the latter groups, the time to death of the non-surviving fish was attenuated. This inter-animal communication of signals has previously been documented for animals exposed to ionizing radiation. Assays of tissues from control, challenged and 'bystander fish exposed to the pathogen showed that a signal as yet unidentified but similar to that seen in bystanders to irradiated fish was being produced. This signal caused a sharp and transient increase in intracellular calcium and a decrease in clonogenicity in a well-characterized reporter assay.
虹鳟（Oncorhynchus mykiss，瓦尔鲍姆）经腹腔注射亚致死剂量的鳗弧菌VIB1进行攻毒，并使其恢复。然后，7天后，将从未接触过该病原体的未感染鱼（指定为“旁观者”鱼）放入同一水箱中。这些鱼与适应（恢复）后的鱼一起游动7天，之后两组以及一个对照组（从未直接接触过病原体或恢复后的鱼）都暴露于致死剂量的VIB1。对照组在3天内死亡率为100%，适应组为47%，与适应组一起游动的未受攻毒旁观者组为60%。在后两组中，未存活鱼的死亡时间都有所延长。此前已有文献记载，暴露于电离辐射的动物之间存在这种信号交流。对对照组、攻毒组和暴露于病原体的“旁观者”鱼的组织检测表明，正在产生一种尚未确定但与受辐照鱼的旁观者中所见信号相似的信号。在一个特征明确的报告检测中，该信号导致细胞内钙急剧短暂增加，并使克隆形成能力下降。
