Zhang Rui, Mei Yong, Liu Yanru, Dai Hao, Xia Hongfang, Zhang Xin, Wu Yukang, Gu Yingying, Peng Xiaowu
School of Public Health, Wuhan University of Science and Technology, Huangjiahu West Road, 430065, Wuhan, Hubei, China.
Center for Environmental Health Research, South China Institute of Environmental Sciences, Ministry of Environmental Protection, Yuancun West Road, 510655, Guangzhou, Guangdong, China.
Biomed Chromatogr. 2015 Oct;29(10):1492-8. doi: 10.1002/bmc.3448. Epub 2015 Mar 10.
The abundant production of methyl tert-butyl ether (MTBE) and its widespread use have led to an increase in the potential for human exposure. This work described a simple, fast, sensitive, reliable and low-cost method for the simultaneous measurement of MTBE and its metabolite, tert-butyl alcohol (TBA) in human serum by headspace solid-phase microextraction gas chromatography-mass spectrometry. Extraction conditions were optimized and 40 °C, 10 min, 250 rpm and 0.3 g NaCl for a 1 mL sample were the optimal conditions. This method showed good analytical performance in terms of sensitivity with limits of detection in serum (1 mL) of 0.03 µg/L for MTBE and 0.05 µg/L for TBA, accuracy (mean recovery values) from 75.8% to 85.8%, precision (relative standard deviations) <10% and sample stability (biodegradation) <10% after 28 days. A verification experiment proved the reproducibility and stability of this method as well. Finally the method was used to detect 212 specimens, and the internal dose levels for MTBE in human serum were presented in China.
甲基叔丁基醚（MTBE）的大量生产及其广泛使用导致人类接触的可能性增加。这项工作描述了一种简单、快速、灵敏、可靠且低成本的方法，用于通过顶空固相微萃取气相色谱 - 质谱法同时测定人血清中的MTBE及其代谢物叔丁醇（TBA）。对萃取条件进行了优化，对于1 mL样品，40°C、10分钟、250转/分钟和0.3 g氯化钠为最佳条件。该方法在灵敏度方面表现出良好的分析性能，血清（1 mL）中MTBE的检测限为0.03 μg/L，TBA的检测限为0.05 μg/L，准确度（平均回收率）为75.8%至85.8%，精密度（相对标准偏差）<10%，28天后样品稳定性（生物降解）<10%。一项验证实验也证明了该方法的重现性和稳定性。最后，该方法用于检测212份标本，并给出了中国人群血清中MTBE的内剂量水平。
