Marciniec B, Wojciechowska B
Acta Pol Pharm. 1989;46(3):248-57.
Two new methods for the determination of active components in the preparations Gardan P (propyphenazone + noramidopyrine) and Pabialgin P (propyphenazone + allobarbital) have been elaborated. The first one is a direct spectrophotometric method based on measurements of absorption at two wave lengths; at lambda = 218 and 270 nm for Pabialgin P, and at lambda = 232 and 260 nm for Gardan P. The second method, the chromatographic-spectrophotometric method has been based on separation of individual components by thin-layer chromatography followed by their spectrophotometric determination. The results obtained by the above two methods were compared with the results given by the titrimetric method according to the producer's standard. Statistic analysis by the Student's and F-Snecodor's tests has shown that all these methods are equally precise but differ in accuracy, sensitivity and selectivity. The spectrophotometric determination of allobarbital has proved to show the highest accuracy (Wz = 1.4), while the acidimetric determination of propyphenazone in the non-aqueous medium has appeared least accurate (Wz = 3.7). Sensitivity of the tested methods was found to range from 3 x 10(-4) g/cm3 for the titrimetric methods to 6 x 10(-6) g/cm3 for direct spectrophotometric measurements. In respect of selectivity, only the chromatographic-spectrophotometric method has proved to be enough selective and to give quite correct results.
已研发出两种测定制剂Gardan P（异丙安替比林+氨基比林）和Pabialgin P（异丙安替比林+异戊巴比妥）中活性成分的新方法。第一种是直接分光光度法，基于在两个波长处测量吸光度；对于Pabialgin P，波长为λ=218和270nm，对于Gardan P，波长为λ=232和260nm。第二种方法，即色谱-分光光度法，是基于通过薄层色谱分离各个成分，然后进行分光光度测定。将上述两种方法得到的结果与根据生产商标准采用滴定法得到的结果进行了比较。通过学生氏检验和F-斯内德科尔检验进行的统计分析表明，所有这些方法的精密度相同，但在准确度、灵敏度和选择性方面存在差异。已证明异戊巴比妥的分光光度测定显示出最高的准确度（Wz=1.4），而异丙安替比林在非水介质中的酸碱滴定法测定准确度最低（Wz=3.7）。发现所测试方法的灵敏度范围从滴定法的3×10⁻⁴g/cm³到直接分光光度测量的6×10⁻⁶g/cm³。就选择性而言，只有色谱-分光光度法已证明具有足够的选择性并能给出相当正确的结果。
