Zweygarth E, Gumm I D, Gray M A, Cheruiyot J K, Webster P, Kaminsky R
Kenya Trypanosomiasis Research Institute, Kikuyu, Kenya.
Acta Trop. 1989 Oct;46(5-6):277-82. doi: 10.1016/0001-706x(89)90040-5.
A stock of Trypanosoma simiae was transformed into procyclic forms at 26 C in a semi-defined maintenance medium. After transformation, the trypanosomes were maintained in a modified Eagle's MEM medium. On day 35 of cultivation, epimastigotes attached to the bottom of the culture flask. From day 44 onwards, metacyclic-like trypanosomes were observed. Subcutaneous injections into pigs of trypanosome suspensions obtained from cultures on day 10 were not infective, whereas culture-derived metacyclics (days 44, 63 and 69) were highly pathogenic.
一批西氏锥虫在26℃的半限定维持培养基中转化为前循环型。转化后，锥虫在改良的伊格尔MEM培养基中培养。在培养的第35天，上鞭毛体附着在培养瓶底部。从第44天起，观察到类循环后期锥虫。第10天从培养物中获得的锥虫悬液皮下注射到猪体内没有感染性，而培养来源的循环后期锥虫（第44、63和69天）具有高度致病性。
