Bradbury Richard S, Barbé Barbara, Jacobs Jan, Jallow Amadou T, Camara Karamo C, Colley Musa, Wegmüller Rita, Jassey Babucarr, Cham Yorro, Baldeh Ignatius, Prentice Andrew
MRC Keneba, MRC The Gambia, Keneba, The Gambia
Department of Clinical Sciences, Institserovarute of Tropical Medicine, Antwerp, Belgium.
Trans R Soc Trop Med Hyg. 2015 May;109(5):334-9. doi: 10.1093/trstmh/trv020. Epub 2015 Mar 10.
Ongoing surveillance of enteric pathogens of public health significance among casual food sellers is undertaken in many resource-limited countries. We report the results of a survey in Kiang West province, The Gambia, and provide an exemplar methodology for such surveys in resource-limited laboratories.
Unpreserved, unrefrigerated stool samples were subjected to Salmonella, Shigella and agar plate culture for rhabditoid nematodes. Direct microscopy, formalin-ethyl acetate concentration and iron-hematoxylin staining was performed later, following preservation.
Of 128 specimens received, no Shigella spp. was recovered, while four serovars of non-typhoidal Salmonella enterica, including Chandans, were isolated. Pathogenic parasitic infections were Necator americanus 10/128 (7.8%), Strongyloides stercoralis 3/128 (2.8%), Blastocystis species 45/128 (35.1%), Entamoeba histolytica complex 19/128 (14.8%) and Giardia intestinalis 4/128 (3.1%). A single case each of Hymenolepis diminuta and S. mansoni infection were detected. In one participant, myxozoan spores identical to those of Myxobolus species were found.
Rare parasitoses and serovars of Salmonella enterica may occur relatively commonly in rural Africa. This paper describes intestinal pathogens found in a cohort of food sellers in such a setting. Furthermore, it describes two parasites rarely recovered from humans and demonstrates the need for methods other than microscopy to detect S. stercoralis infections.
许多资源有限的国家都在对街头食品摊贩中具有公共卫生意义的肠道病原体进行持续监测。我们报告了在冈比亚的下江省进行的一项调查结果，并为资源有限的实验室进行此类调查提供了一个示例方法。
未保存、未冷藏的粪便样本进行沙门氏菌、志贺氏菌和类圆线虫琼脂平板培养。保存后，随后进行直接显微镜检查、福尔马林 - 乙酸乙酯浓缩和铁苏木精染色。
在收到的128份标本中，未分离出志贺氏菌属，而分离出了包括昌丹斯在内的四种非伤寒沙门氏菌血清型。致病性寄生虫感染有美洲板口线虫10/128（7.8%）、粪类圆线虫3/128（2.8%）、芽囊原虫属45/128（35.1%）、溶组织内阿米巴复合体19/128（14.8%）和肠贾第虫4/128（3.1%）。检测到各1例微小膜壳绦虫和曼氏血吸虫感染。在一名参与者中，发现了与黏液孢子虫属相同的黏液虫孢子。
非洲农村地区可能相对普遍地出现罕见的寄生虫病和非伤寒沙门氏菌血清型。本文描述了在这样一个环境中的一组食品摊贩中发现的肠道病原体。此外，本文描述了两种很少从人类中分离出的寄生虫，并证明了除显微镜检查外还需要其他方法来检测粪类圆线虫感染。
