Januszkiewicz A, Wołkowicz T, Chróst A, Szych J
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.
Lett Appl Microbiol. 2015 Jun;60(6):605-8. doi: 10.1111/lam.12413. Epub 2015 Apr 8.
Shiga toxin-producing Escherichia coli (STEC) O26 infections can be comparable with STEC O157 infections in severity of the acute haemolytic-uremic syndrome HUS and long-term sequelae. Among O26 STEC isolates, highly virulent clone O26:H11/H- Sequence Type 29 (ST 29) emerged in Germany in mid-1990s and spread to European countries. However, up to date, no STEC O26:H11/H- belonging to ST29 has been documented in Poland. In this study, we determined the relationship and clonal structure, stx genotypes, plasmid gene profiles and antimicrobial resistance of nine human STEC O26:H11/H- strains from human patients in Poland between 1996 and 2014. Of the 9 human STEC O26:H11/H- strains, two belonged to ST29 and were isolated from two children with HUS and renal failure with sepsis respectively. These strains showed the molecular characteristics of the emerging human-pathogenic ST29 clone (stx1-, stx2a+, eae+, ehxA+, etpD+, katP-, espP-). The remaining STEC O26:H11/H- strains examined in this study, belonged to ST21, with plasmid genes profiles frequently reported in ST21 strains in Europe. STEC O26 infections with serious human health consequences highlight the need of continuous surveillance of non-O157 STEC and implementation of the diagnostic approaches focused on their detection. Significance and impact of the study: These study provides the first data on the occurrence of emerging Shiga toxin-producing Escherichia coli O26:H11 ST 29 clone in human patients in Poland. Those strains show the molecular characteristics of highly virulent new ST29 pathotype (stx1-, stx2a+, eae+ ehxA+, etpD+, katP-, espP-). These results demonstrated prompt efforts to implement diagnostic approaches detection of those pathogen in the European countries.
产志贺毒素大肠杆菌（STEC）O26感染在急性溶血尿毒综合征（HUS）的严重程度和长期后遗症方面可能与STEC O157感染相当。在O26 STEC分离株中，高毒力克隆O26:H11/H-序列型29（ST29）于20世纪90年代中期在德国出现，并传播到欧洲国家。然而，截至目前，波兰尚未有属于ST29的STEC O26:H11/H-的记录。在本研究中，我们确定了1996年至2014年间从波兰人类患者中分离出 的9株人类STEC O26:H11/H-菌株之间的关系和克隆结构、stx基因型、质粒基因图谱及抗菌药物耐药性。在这9株人类STEC O26:H11/H-菌株中，有两株属于ST29，分别从两名患有HUS和伴有败血症的肾衰竭儿童中分离得到。这些菌株表现出新兴的人类致病ST29克隆的分子特征（stx1-、stx2a+、eae+、ehxA+、etpD+、katP-、espP-）。本研究中检测的其余STEC O26:H11/H-菌株属于ST21，其质粒基因图谱与欧洲ST21菌株中经常报道的一致。具有严重人类健康后果的STEC O26感染凸显了持续监测非O157 STEC以及实施专注于其检测的诊断方法的必要性。研究的意义和影响：本研究提供了波兰人类患者中出现的产志贺毒素大肠杆菌O26:H11 ST29克隆的首批数据。这些菌株表现出高毒力新ST29致病型的分子特征（stx1-、stx2a+、eae+、ehxA+、etpD+、katP-、espP-）。这些结果表明欧洲国家已迅速努力实施针对这些病原体的诊断检测方法。
