The tolerance of chickens to acute heat stress, evaluated by the time required to reach the critical body temperature (Tr) of 44.5 degrees C, was markedly enhanced as the period of fasting was extended. 2. Fasting reduced the rates of heat-induced changes in blood acid-base and electrolyte status. 3. Changes in Tr were correlated with changes in blood pH, pCO2, [Cl-] and [Pi] but not with changes in [Na+] or [K+]. 4. Blood acid-base and electrolyte status were related to Tr rather than time of exposure to heat stress.