Department of Biochemistry, Microbiology and Bio-Informatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6 ; Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3.
J Immunol Res. 2014;2014:635108. doi: 10.1155/2014/635108. Epub 2014 Dec 23.
B lymphocyte differentiation into long-lived plasma cells is the keystone event for the production of long-term protective antibodies. CD40-CD154 and CD27-CD70 interactions are involved in human B lymphocyte differentiation into CD38(hi)CD138(+) cells in vivo as well as in vitro. In this study, we have compared these interactions in their capacity to drive switched-memory B lymphocytes differentiation into CD38(hi)CD138(+) plasma cells. The targeted B lymphocytes were isolated from human peripheral blood, expanded for 19 days, and then submitted to CD70 or CD154 interactions for 14 days. The expanded B lymphocytes were constitutively expressing CD39, whereas CD31's expression was noticed only following the in vitro differentiation step (day 5) and was exclusively present on the CD38(hi) cell population. Furthermore, the generated CD38(hi)CD138(+) cells showed a higher proportion of CD31(+) cells than the CD38(hi)CD138(-) cells. Besides, analyses done with human blood and bone marrow plasma cells showed that in vivo and de novo generated CD38(hi)CD138(+) cells have a similar CD31 expression profile but are distinct according to their reduced CD39 expression level. Overall, we have evidences that in vitro generated plasma cells are heterogeneous and appear as CD39(+) precursors to the ones present in bone marrow niches.
B 淋巴细胞分化为长寿浆细胞是产生长期保护性抗体的关键事件。CD40-CD154 和 CD27-CD70 相互作用参与体内和体外人类 B 淋巴细胞向 CD38(hi)CD138(+)细胞的分化。在这项研究中，我们比较了这些相互作用在驱动已转换的记忆 B 淋巴细胞分化为 CD38(hi)CD138(+)浆细胞的能力。靶向 B 淋巴细胞从人外周血中分离出来，扩增 19 天，然后接受 CD70 或 CD154 相互作用 14 天。扩增的 B 淋巴细胞持续表达 CD39，而 CD31 的表达仅在体外分化阶段（第 5 天）被注意到，并且仅存在于 CD38(hi)细胞群上。此外，生成的 CD38(hi)CD138(+)细胞比 CD38(hi)CD138(-)细胞具有更高比例的 CD31(+)细胞。此外，对人血液和骨髓浆细胞的分析表明，体内和新生成的 CD38(hi)CD138(+)细胞具有相似的 CD31 表达谱，但根据其降低的 CD39 表达水平而不同。总的来说，我们有证据表明，体外生成的浆细胞是异质的，并且作为骨髓龛中存在的 CD39(+)前体出现。
