Brunet S, Casals T, Madoz P, Aventín A, Muñiz E, Baiget M
Med Clin (Barc). 1989 Dec 16;93(20):765-71.
After allogeneic bone marrow transplantation (BMT), patient hematopoietic and lymphoid cells are replaced by cells derived from the donor marrow. To document and characterize successful engraftment, host and donor cells must be distinguished from each other. We have used DNA sequence polymorphism analysis in 6 patients, at times varying, to determine reliably the host or donor origin of posttransplant cell populations and to compare these results with those obtained using red blood cell antigens and cytogenetics. Initially full engraftment was documented in all patients. In 1 patient a mixed lymphohematopoietic chimerism was documented 6 months after BMT and it reverted to donor hematopoiesis at 1 year post BMT. Posttransplant leukemic relapse was studied in two patients and shown to be of host origin in both cases. The DNA restriction fragment length polymorphisms (RFLP), are a powerful tool for the documentation of engraftment after BMT, to document mixed lymphohematopoietic chimerism and for the evaluation of leukemic relapse.
异基因骨髓移植（BMT）后，患者的造血细胞和淋巴细胞被来自供体骨髓的细胞所取代。为了记录和表征成功的植入，必须将宿主细胞和供体细胞区分开来。我们在6例患者中使用了DNA序列多态性分析，时间各不相同，以可靠地确定移植后细胞群体的宿主或供体来源，并将这些结果与使用红细胞抗原和细胞遗传学获得的结果进行比较。最初，所有患者均记录到完全植入。1例患者在BMT后6个月记录到混合性淋巴造血嵌合体，在BMT后1年恢复为供体造血。对2例患者进行了移植后白血病复发研究，结果显示两例均为宿主来源。DNA限制性片段长度多态性（RFLP）是记录BMT后植入、记录混合性淋巴造血嵌合体以及评估白血病复发的有力工具。
