Peredo H A, Lee H, Donoso A S, Andrade V, Sánchez Eluchans N, Puyó A M
CONICET, Cátedra Anatomía Histología and INFIBIOC, Junin 956, Buenos Aires, Argentina; Fac Farm y Bioq UBA, Cátedra Anatomía Histología and INFIBIOC, Junin 956, Buenos Aires, Argentina.
Auton Autacoid Pharmacol. 2015 Apr;34(3-4):35-40. doi: 10.1111/aap.12021. Epub 2015 Mar 12.
In the rat, a high-fat (HF) plus fructose (F) diet produces cardiovascular and metabolic alterations that resemble human metabolic syndrome. Prostanoids (PR), cyclo-oxygenase-derived arachidonic acid metabolites, have vasoactive properties and mediate inflammation. The aim of this study was to analyse the effect of a HF+F diet on blood pressure (BP), metabolic parameters and mesenteric vascular bed PR production in male Sprague-Dawley rats. Four groups were studied over 9 weeks (n = 6 each): control (C), standard diet (SD) and tap water to drink; F+SD and 10% w/v F solution to drink; HF 50% (w/w) bovine fat added to SD and tap water; and HFF, both treatments. PR were determined by HPLC. Blood pressure was elevated in all experimental groups. Triglyceridaemia, insulinaemia and HOMA-IR were increased in the F and HF groups. HF+F animals showed elevated glycaemia, insulinaemia, HOMA-IR and triglyceridaemia. F decreased the vasodilator prostanoids PGI2 and PGE2 in the mesenteric vascular bed. Body weight was not significantly altered. In HFF, production of PGE2 , PGF2 alpha and TXB2 was elevated. The increased BP in HF and HFF could be partly attributed to the imbalance in vascular PR production towards vasoconstrictors. On the other hand, this dietary modification could induce inflammation, which would explain the elevation of PGE2 . In the F group, hypertension could be related to decreased vasodilator PRs. The simultaneous administration of HF and F in the rat produces deleterious effects greater than observed when treatments are applied separately.
在大鼠中，高脂（HF）加果糖（F）饮食会引发心血管和代谢改变，类似于人类代谢综合征。前列腺素（PR）是环氧化酶衍生的花生四烯酸代谢产物，具有血管活性并介导炎症。本研究的目的是分析HF+F饮食对雄性Sprague-Dawley大鼠血压（BP）、代谢参数和肠系膜血管床PR产生的影响。对四组大鼠进行了为期9周的研究（每组n = 6）：对照组（C），给予标准饮食（SD）并饮用自来水；F+SD组，饮用含10% w/v F的溶液；HF组，在SD中添加50%（w/w）牛脂并饮用自来水；以及HFF组，同时给予上述两种处理。通过高效液相色谱法测定PR。所有实验组的血压均升高。F组和HF组的甘油三酯血症、胰岛素血症和HOMA-IR均升高。HF+F组动物的血糖、胰岛素血症、HOMA-IR和甘油三酯血症均升高。F降低了肠系膜血管床中的血管舒张前列腺素PGI2和PGE2。体重没有显著变化。在HFF组中，PGE2、PGF2α和TXB2的产生增加。HF组和HFF组血压升高可能部分归因于血管PR产生向血管收缩剂的失衡。另一方面，这种饮食改变可能会诱发炎症，这可以解释PGE2的升高。在F组中，高血压可能与血管舒张PR的减少有关。在大鼠中同时给予HF和F产生的有害影响大于单独应用这些处理时观察到的影响。
