Hinney Barbara, Ederer Christina, Stengl Carina, Wilding Katrin, Štrkolcová Gabriela, Harl Josef, Flechl Eva, Fuehrer Hans-Peter, Joachim Anja
Institute of Parasitology, Department of Pathobiology, Vetmeduni Vienna, Veterinärplatz 1, 1210, Wien, Austria,
Parasitol Res. 2015 May;114(5):2003-6. doi: 10.1007/s00436-015-4408-0. Epub 2015 Mar 13.
Domestic cats can be infected with a variety of enteric protozoa. Genotyping of protozoan species, especially Giardia as the most common, can improve assessment of their relevance as zoonotic agents. For an overview on the occurrence of feline enteric protozoa, 298 faecal samples of cats from private households, catteries and animal shelters in Austria were collected. All samples were examined by flotation and using a rapid test for Giardia (FASTest). For the detection of Tritrichomonas blagburni, freshly voided faeces (n = 40) were processed using a commercial culturing system (InPouch TF-Feline). Genotyping was done at the β-giardin gene loci (each sample) and triosephosphate isomerase gene loci (positive samples) for Giardia and at the 18S rRNA gene (positive samples) for Cryptosporidium. Thirty-seven samples (12.4%) were positive for Giardia by flotation and/or using a rapid test. Cryptosporidium was present in 1.7%, Cystoisospora in 4.0%, Sarcocystis in 0.3% and T. blagburni in 2.5% of the samples. Genotyping revealed Giardia cati, the potentially zoonotic Giardia duodenalis and Cryptosporidium felis. Most of the infected cats had no diarrhoea. Cats from shelters were significantly more often infected than owned cats (p = 0.01). When comparing Giardia detection methods, the rapid test had a higher sensitivity than flotation. Polymerase chain reaction (PCR) results were mostly independent from the other two tests.
家猫可能感染多种肠道原生动物。对原生动物物种进行基因分型，尤其是对最常见的贾第虫进行基因分型，有助于更好地评估它们作为人畜共患病原体的相关性。为了概述猫肠道原生动物的感染情况，收集了来自奥地利私人家庭、猫舍和动物收容所的298份猫粪便样本。所有样本均通过浮选法和使用贾第虫快速检测试剂盒（FASTest）进行检测。对于布拉氏三毛滴虫的检测，使用商业培养系统（InPouch TF-Feline）对40份新鲜排出的粪便进行处理。对贾第虫在β-贾第虫基因位点（每个样本）和磷酸丙糖异构酶基因位点（阳性样本）进行基因分型，对隐孢子虫在18S rRNA基因（阳性样本）进行基因分型。通过浮选法和/或使用快速检测试剂盒，37份样本（12.4%）的贾第虫检测呈阳性。1.7%的样本中存在隐孢子虫，4.0%存在等孢球虫，0.3%存在肉孢子虫，2.5%存在布拉氏三毛滴虫。基因分型显示存在猫贾第虫、具有潜在人畜共患性的十二指肠贾第虫和猫隐孢子虫。大多数受感染的猫没有腹泻症状。收容所的猫比家养猫感染的频率明显更高（p = 0.01）。在比较贾第虫检测方法时，快速检测试剂盒的灵敏度高于浮选法。聚合酶链反应（PCR）结果大多与其他两种检测方法无关。
