Gómez-Romano María Teresa, Rodríguez-Iglesias Manuel Antonio, Galán-Sánchez Fátima
C.E.P. Salus Infirmorum, 11001 Cádiz, Spain.
Servicio de Microbiología, Hospital Universitario Puerta del Mar, 11009 Cádiz, Spain.
Microorganisms. 2024 Apr 30;12(5):918. doi: 10.3390/microorganisms12050918.
spp. and Microsporidia are opportunistic microorganisms with remarkable zoonotic transmission potential due to their capacity to infect humans and animals. The aim of this study was to evaluate the prevalence of these microorganisms in stool samples of animal and human origin. In total, 369 stool samples (205 from human patients with diarrhea and 164 of animal origin) were included in the study. spp. and Microsporidia presence were determined by using multiplex nested PCR. Positive results were analyzed by using Sanger sequencing of the amplicon, utilizing BLASTN and ClustalX software to confirm identification. spp. were found in 0.97% and 4.26% of human and animal samples, respectively. was detected in human and animal stools in 6.82% and 3.05% of the samples, respectively. No associations were found when analyzing the presence of spp. and and the demographic and clinical variables of patients and animals. This study demonstrates the presence of these microorganisms in human and animal samples from different species, and the most interesting findings are the detection of spp. in pets (e.g., rodents) that are not usually included in this type of study, and the identification of in patients with diarrhea without underlying disease.
某些物种和微孢子虫是机会性微生物，由于它们能够感染人类和动物，因此具有显著的人畜共患病传播潜力。本研究的目的是评估这些微生物在动物和人类来源粪便样本中的流行情况。该研究共纳入了369份粪便样本（205份来自腹泻人类患者，164份为动物来源）。通过多重巢式PCR确定某些物种和微孢子虫的存在情况。利用扩增子的桑格测序对阳性结果进行分析，使用BLASTN和ClustalX软件来确认鉴定结果。某些物种分别在0.97%的人类样本和4.26%的动物样本中被发现。分别在6.82%的人类粪便样本和3.05%的动物粪便样本中检测到了（原文此处未明确的微生物名称）。在分析某些物种和（原文此处未明确的微生物名称）的存在情况与患者和动物的人口统计学及临床变量之间时，未发现相关性。本研究证明了这些微生物在来自不同物种的人类和动物样本中的存在，最有趣的发现是在通常不纳入此类研究的宠物（如啮齿动物）中检测到了某些物种，以及在无基础疾病的腹泻患者中鉴定出了（原文此处未明确的微生物名称）。
