对多能干细胞施加剪切应力。
Applying Shear Stress to Pluripotent Stem Cells.
作者信息
Wolfe Russell P, Guidry Julia B, Messina Stephanie L, Ahsan Tabassum
机构信息
Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
出版信息
Methods Mol Biol. 2016;1341:377-89. doi: 10.1007/7651_2015_210.
Abstract
Thorough understanding of the effects of shear stress on stem cells is critical for the rationale design of large-scale production of cell-based therapies. This is of growing importance as emerging tissue engineering and regenerative medicine applications drive the need for clinically relevant numbers of both pluripotent stem cells (PSCs) and cells derived from PSCs. Here, we describe the use of a custom parallel plate bioreactor system to impose fluid shear stress on a layer of PSCs adhered to protein-coated glass slides. This system can be useful both for basic science studies in mechanotransduction and as a surrogate model for bioreactors used in large-scale production.
摘要
深入了解剪切应力对干细胞的影响对于合理设计基于细胞的疗法的大规模生产至关重要。随着新兴的组织工程和再生医学应用推动对临床相关数量的多能干细胞（PSC）以及源自PSC的细胞的需求，这一点变得越来越重要。在这里，我们描述了使用定制的平行板生物反应器系统对粘附在蛋白包被的载玻片上的一层PSC施加流体剪切应力。该系统对于机械转导的基础科学研究以及作为大规模生产中使用的生物反应器的替代模型都可能有用。
相似文献
1
Applying Shear Stress to Pluripotent Stem Cells.对多能干细胞施加剪切应力。
Methods Mol Biol. 2016;1341:377-89. doi: 10.1007/7651_2015_210.
2
Teratoma formation of human embryonic stem cells in three-dimensional perfusion culture bioreactors.三维灌注培养生物反应器中人胚胎干细胞的畸胎瘤形成。
J Tissue Eng Regen Med. 2013 Sep;7(9):729-41. doi: 10.1002/term.1467. Epub 2012 Mar 21.
3
An intermittent rocking platform for integrated expansion and differentiation of human pluripotent stem cells to cardiomyocytes in suspended microcarrier cultures.一种用于在悬浮微载体培养中实现人多能干细胞向心肌细胞整合性扩增与分化的间歇性摇摆平台。
Stem Cell Res. 2014 Sep;13(2):202-13. doi: 10.1016/j.scr.2014.06.002. Epub 2014 Jun 27.
4
Scalable Expansion of Pluripotent Stem Cells.多能干细胞的可扩展扩增
Adv Biochem Eng Biotechnol. 2018;163:23-37. doi: 10.1007/10_2017_26.
5
Expansion and long-term maintenance of induced pluripotent stem cells in stirred suspension bioreactors.搅拌悬浮生物反应器中诱导多能干细胞的扩增和长期维持。
J Tissue Eng Regen Med. 2012 Jun;6(6):462-72. doi: 10.1002/term.450. Epub 2011 Jul 14.
6
Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors.人诱导多能干细胞在搅拌悬浮生物反应器中的扩增
Methods Mol Biol. 2016;1502:53-61. doi: 10.1007/7651_2015_311.
7
Large-scale expansion of pluripotent human embryonic stem cells in stirred-suspension bioreactors.大规模扩增悬浮搅拌培养的人胚胎干细胞。
Tissue Eng Part C Methods. 2010 Aug;16(4):573-82. doi: 10.1089/ten.TEC.2009.0228.
8
Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies.用于从多能干细胞衍生的胚状体进行可扩展心肌分化的新型生物反应器平台
Methods Mol Biol. 2016;1502:169-79. doi: 10.1007/7651_2016_341.
9
Making cardiomyocytes: how mechanical stimulation can influence differentiation of pluripotent stem cells.制造心肌细胞：机械刺激如何影响多能干细胞的分化
Biotechnol Prog. 2013 Sep-Oct;29(5):1089-96. doi: 10.1002/btpr.1794. Epub 2013 Sep 12.
10
Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors.优化的低接种密度传代培养人诱导多能干细胞，以在垂直旋转式生物反应器中生成临床相关数量的细胞。
Stem Cells Transl Med. 2020 Sep;9(9):1036-1052. doi: 10.1002/sctm.19-0406. Epub 2020 May 22.
引用本文的文献
1
Robust bioprocess design and evaluation of commercial media for the serial expansion of human induced pluripotent stem cell aggregate cultures in vertical-wheel bioreactors.在垂直轮生物反应器中对人诱导多能干细胞集落培养进行连续扩增的商业培养基的稳健生物工艺设计和评估。
Stem Cell Res Ther. 2024 Jul 29;15(1):232. doi: 10.1186/s13287-024-03819-9.
2
Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells.中枢神经系统组织工程：神经干细胞/祖细胞与导电生物材料的界面。
Adv Healthc Mater. 2022 Apr;11(7):e2101577. doi: 10.1002/adhm.202101577. Epub 2021 Dec 16.
本文引用的文献
1
Bioreactor design for clinical-grade expansion of stem cells.用于临床级干细胞扩增的生物反应器设计。
Biotechnol J. 2013 Jun;8(6):644-54. doi: 10.1002/biot.201200373. Epub 2013 Apr 26.
2
Shear stress during early embryonic stem cell differentiation promotes hematopoietic and endothelial phenotypes.早期胚胎干细胞分化过程中的切应力促进造血和内皮表型。
Biotechnol Bioeng. 2013 Apr;110(4):1231-42. doi: 10.1002/bit.24782. Epub 2013 Feb 15.
3
Effects of shear stress on germ lineage specification of embryonic stem cells.切应力对胚胎干细胞生殖系特化的影响。
Integr Biol (Camb). 2012 Oct;4(10):1263-73. doi: 10.1039/c2ib20040f.
4
Shear stress influences the pluripotency of murine embryonic stem cells in stirred suspension bioreactors.切应力影响搅拌悬浮生物反应器中鼠胚胎干细胞的多能性。
J Tissue Eng Regen Med. 2014 Apr;8(4):268-78. doi: 10.1002/term.1518. Epub 2012 Jun 1.
5
Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask.大规模扩增和利用多能干细胞进行再生医学目的：超越 T 瓶。
Regen Med. 2012 Jan;7(1):71-84. doi: 10.2217/rme.11.101.
6
Fluid shear stress primes mouse embryonic stem cells for differentiation in a self-renewing environment via heparan sulfate proteoglycans transduction.流体切应力通过硫酸乙酰肝素蛋白聚糖转导使处于自我更新环境中的小鼠胚胎干细胞向分化方向启动。
FASEB J. 2011 Apr;25(4):1208-17. doi: 10.1096/fj.10-168971. Epub 2010 Dec 23.
7
Fluid shear stress promotes an endothelial-like phenotype during the early differentiation of embryonic stem cells.流体切应力在胚胎干细胞的早期分化过程中促进内皮样表型的形成。
Tissue Eng Part A. 2010 Nov;16(11):3547-53. doi: 10.1089/ten.TEA.2010.0014. Epub 2010 Aug 28.
8
Hydrodynamic modulation of embryonic stem cell differentiation by rotary orbital suspension culture.旋转轨道悬浮培养对胚胎干细胞分化的流体动力学调节。
Biotechnol Bioeng. 2010 Feb 15;105(3):611-26. doi: 10.1002/bit.22578.
9
Scalable stirred-suspension bioreactor culture of human pluripotent stem cells.可扩展搅拌悬浮生物反应器培养人多能干细胞。
Tissue Eng Part A. 2010 Feb;16(2):405-21. doi: 10.1089/ten.tea.2009.0454.
10
Biomechanical forces promote embryonic haematopoiesis.生物力学力促进胚胎造血。
Nature. 2009 Jun 25;459(7250):1131-5. doi: 10.1038/nature08073. Epub 2009 May 13.
Zotero 插件