Gylmiyarova F N, Radomskaya V M, Gusyakova O A, Ryskina E A, Kolotyeva N A, Shahnovich E A, Nefedova N S, Baisheva G M, Pervova Yu Y, Pleten A P
Biomed Khim. 2015 Jan-Feb;61(1):132-40. doi: 10.18097/pbmc20156101132.
Using the ABO antibody-antigen model the influence of natural metabolite pyruvate on the antibody interaction with of erythrocyte antigens, defining their group specificity has been investigated. Before agglutination reaction erythrocytes of A(II)-AB(IV) blood groups, monoclonal anti-A and anti-B antibodies were incubated with sodium pyruvate. Visualization of agglutinates was performed by means of flow cytometry and laser scanning confocal microscopy. Computer-aided prediction of the spectrum of biological activity of pyruvate by a PASS program proposed major regulatory pathways, in which pyruvate may be involved. It has been demonstrated that pyruvate can regulate the intensity of antigen-antibody interaction. These results suggest the possibility of using small molecules, for example pyruvate, as molecular probes and prospects of the use of erythrocytes with antigenic determinants of the ABO system expressed on their membranes for studies of protein-protein interactions due to convenient visualization and possibility of quantitative evaluation of this process.
利用ABO抗体-抗原模型，研究了天然代谢产物丙酮酸对抗体与红细胞抗原相互作用的影响，确定了它们的血型特异性。在凝集反应前，将A(II)-AB(IV)血型的红细胞、单克隆抗A和抗B抗体与丙酮酸钠一起孵育。通过流式细胞术和激光扫描共聚焦显微镜对凝集物进行可视化。通过PASS程序对丙酮酸生物活性谱进行计算机辅助预测，提出了丙酮酸可能参与的主要调控途径。已证明丙酮酸可调节抗原-抗体相互作用的强度。这些结果表明，小分子（如丙酮酸）可作为分子探针，以及利用膜上表达有ABO系统抗原决定簇的红细胞进行蛋白质-蛋白质相互作用研究的前景，这是由于该过程可视化方便且具有定量评估的可能性。
