State Institution "Institute of Pathology of the Spine and Joints named after prof. M. I. Sitenko of the Academy of Medical Sciences of Ukraine", Kharkov.
State Enterprise "Ukrainian Scientific Pharmacopoeial Center for the Quality of Medicines", Kharkov.
Arch Razi Inst. 2024 Feb 1;79(1):55-67. doi: 10.32592/ARI.2024.79.1.55. eCollection 2024 Feb.
The biosynthesis of agglutinogenic and adsorbing groups A and B glycotopes of the erythrocyte's membrane is mediated by the activity of specific glycosyltransferases. This study aimed to assess the nature of the biosynthesis of A and B antigenic glycotopes, depending on the pH of the medium during the cultivation of erythrocytes, and the antigenic (transferase) characteristics of the donor serum of the other group. Monoclonal antibodies (Mabs) were obtained from IGBRL under Program IV of the International Workshop on Monoclonal Antibodies and Red Blood Cell Antigens. Biosynthesis was performed using erythrocytes, fresh serum, medium 199, and an antibiotic solution. The agglutinogenic characteristics of 11 out of 33 samples changed by the end of the cultivation period due to the acquisition of additional agglutinogen corresponding to the donor serum. None of the samples lost their inherent agglutinogen due to its absence in the donor serum. Four of six samples of O(I) erythrocytes acquired the ability to be agglutinated by anti-A reagents, especially by the polyclonal anti-A, and the manifestation of agglutination depended on the reaction time. Two of the three samples with initial A(II) agglutinogenic specificity added to the donor serum with Bc'+ characteristic of the erythrocytes acquired this characteristic. However, none of the five A(II)Ac'+ samples cultured in the serum of Ac'-O(I)Ac'-Bc'+ and O(I)Ac'-Bc'- donors lost their inherent earlier Ac'+ characteristic. The investigation of the inhibitory ability of alkaline and acidic glycoconjugates isolated from membranes revealed that alkaline Alp-00 and Alp-1 glycotopes isolated from glycolipids had the highest inhibitory activity, and the degree of inhibition of polyclonal anti-A antibodies was even higher than that of monovalent BRIC-131. This study showed the possibility of the biosynthesis of specific non-agglutinogenic A and B glycotopes under the influence of a different group's serum as a source of the corresponding transferase.
红细胞膜上的凝集原和吸附基团 A 和 B 糖基的生物合成是由特定糖基转移酶的活性介导的。本研究旨在评估在红细胞培养过程中介质 pH 值以及供体血清的抗原（转移酶）特性对 A 和 B 抗原性糖基合成的影响。单克隆抗体（Mabs）是由 IGBRL 在国际单克隆抗体和红细胞抗原研讨会上的项目 IV 下获得的。生物合成使用红细胞、新鲜血清、199 培养基和抗生素溶液进行。由于获得了与供体血清相应的额外凝集原，33 个样本中的 11 个在培养期末的凝集原特性发生了变化。由于供体血清中不存在，没有一个样本失去其固有的凝集原。6 个 O(I)红细胞样本中的 4 个获得了被抗 A 试剂凝集的能力，特别是多克隆抗 A，凝集的表现取决于反应时间。在初始 A(II)凝集原特异性的 3 个样本中，有 2 个添加了具有红细胞 Bc'+特性的供体血清，获得了这种特性。然而，在 Ac'-O(I)Ac'-Bc'+和 O(I)Ac'-Bc'-供体血清中培养的 5 个 A(II)Ac'+样本中，没有一个失去了其固有的早期 Ac'+特性。对从膜中分离的碱性和酸性糖缀合物的抑制能力的研究表明，从糖脂中分离的碱性 Alp-00 和 Alp-1 糖基具有最高的抑制活性，多克隆抗 A 抗体的抑制程度甚至高于单价 BRIC-131。这项研究表明，在不同组血清作为相应转移酶来源的影响下，有可能合成特定的非凝集原性 A 和 B 糖基。
