用于膜蛋白定向封装的蛋白脂质体。
Proteo-lipobeads for the oriented encapsulation of membrane proteins.
作者信息
Frank Pinar, Siebenhofer Bernhard, Hanzer Theresa, Geiss Andreas F, Schadauer Florian, Reiner-Rozman Ciril, Durham Bill, Loew Leslie M, Ludwig Bernd, Richter Oliver-M H, Nowak Christoph, Naumann Renate L C
机构信息
Biosensor Technologies, Austrian Institute of Technology GmbH, AIT, Donau-City Str. 1, 1220 Vienna, Austria.
Department of Chemistry, University of Arkansas, Fayetteville, AR 72701.
出版信息
Soft Matter. 2015 Apr 21;11(15):2906-2908. doi: 10.1039/c4sm02646b.
Abstract
As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.
摘要
作为活细胞的替代物，蛋白脂质体被呈现出来，它将功能性膜蛋白以严格的方向包裹在脂质双层中。测定可以像在活细胞上一样进行，例如使用荧光测量。作为概念验证，我们已经证明了质子通过细胞色素c氧化酶的运输。
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