Rogniaux Hélène, Pavlovic Marija, Lupi Roberta, Lollier Virginie, Joint Mathilde, Mameri Hamza, Denery Sandra, Larré Colette
INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
Proteomics. 2015 May;15(10):1736-45. doi: 10.1002/pmic.201400416.
Food allergy has become a major health issue in developed countries, therefore there is an urgent need to develop analytical methods able to detect and quantify with a good sensitivity and reliability some specific allergens in complex food matrices. In this paper, we present a targeted MS/MS approach to compare the relative abundance of the major recognized wheat allergens in the salt-soluble (albumin/globulin) fraction of wheat grains. Twelve allergens were quantified in seven wheat varieties, selected from three Triticum species: T. aestivum (bread wheat), T. durum (durum wheat), and T. monococcum. The allergens were monitored from one or two proteotypic peptides and their relative abundance was deduced from the intensity of one fragment measured in MS/MS. Whereas the abundance of some of the targeted allergens was quite stable across the genotypes, others like alpha-amylase inhibitors showed clear differences according to the wheat species, in accordance with the results of earlier functional studies. This study enriches the scarce knowledge available on allergens content in wheat genotypes, and brings new perspectives for food safety and plant breeding.
食物过敏已成为发达国家的一个主要健康问题，因此迫切需要开发能够在复杂食物基质中以良好的灵敏度和可靠性检测和定量某些特定过敏原的分析方法。在本文中，我们提出了一种靶向MS/MS方法，以比较小麦籽粒盐溶性（白蛋白/球蛋白）组分中主要公认的小麦过敏原的相对丰度。在从三个小麦种中选出的七个小麦品种中对12种过敏原进行了定量，这三个小麦种分别是：普通小麦（面包小麦）、硬粒小麦（硬质小麦）和一粒小麦。从一种或两种蛋白型肽段监测过敏原，并根据在MS/MS中测得的一个片段的强度推断它们的相对丰度。虽然一些靶向过敏原的丰度在不同基因型间相当稳定，但其他过敏原如α-淀粉酶抑制剂根据小麦品种的不同表现出明显差异，这与早期功能研究的结果一致。本研究丰富了关于小麦基因型中过敏原含量的稀缺知识，并为食品安全和植物育种带来了新的视角。
